The antifungal activity of the essential oil of (Benth.) Govaerts was investigated against several strains of spp. including clinical isolates and reference strains. Antifungal efficacy was evaluated by determining minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The chemical composition of the essential oil, characterized by gas chromatography-mass spectrometry (GC/MS), revealed pulegone (18.5%), thymol (11.0%), and isomenthone (10.0%) as the primary constituents. essential oil (EO) demonstrated broad-spectrum anticandidal activity against all tested spp., with MIC values ranging from 16 to 64 μg/mL and MFC values from 16 to 128 μg/mL. The EO exhibited potent fungicidal activity (MFC/MIC ratio ≤2) against several strains, notably (MIC and MFC of 16 μg/mL), and also showed efficacy against and . Among the major components, thymol generally displayed the lowest MIC values (32-64 μg/mL). Molecular docking studies further suggested thymol as a key contributor to the oil's antifungal effect, showing strong binding affinities to virulence proteins Als9-2 and the enzyme CYP51. Significantly, the essential oil outperformed amphotericin B against all tested clinical isolates. Overall, EO exhibits significant fungistatic and fungicidal activity against pathogenic species without affecting normal cell viability. These findings, supported by chemical characterization and analysis of its major components like thymol, highlight its potential as a source of novel natural antifungal agents.