Morgan R L, Castles T R, Zwicker G M, Taylor D O
Toxicol Pathol. 1985;13(3):185-91. doi: 10.1177/019262338501300304.
Two insecticide formulations were evaluated for skin irritation using albino rabbits (Stauffland-White strain). While the active ingredient alone produced only mild skin irritation, corn grit formulation produced severe skin irritation. Corn grit from three sources (A, B, and C) was similarly evaluated for skin irritation and at 24 hr, all samples produced erythema and edema of both the abraded and nonabraded test sites. Eschar was observed in 72 hr in about half of the rabbits and persisted through termination on the 7th day. Histologic examination of skin specimens revealed that all three corn grit samples produced epidermatitis. In addition, rabbits exposed to corn grit from two sources (A and B), developed moderate focal to severe diffuse suppurative necrotizing folliculitis and dermatitis. Large tubular branching nonseptate hyphae compatible with the Mucorales species were seen in hair follicle micropustules of rabbits treated with corn grit from sources A and B. Mycologic culture techniques applied to corn grit from each source revealed a potential pathogen in the genus Rhizopus isolated from samples from sources A and C but not B. The skin irritation test involved application of test material covered with gauze to both abraded and nonabraded skin. Rubber damming was placed over the gauze and secured with tape. After 24-hr exposure all bandaging and visible test material were removed. Skin irritation was evaluated immediately after removal and then periodically until termination at 7 days.
使用白化兔（施陶夫兰 - 怀特品系）对两种杀虫剂制剂进行了皮肤刺激性评估。仅活性成分单独使用时仅产生轻度皮肤刺激，而玉米粗粒制剂则产生严重的皮肤刺激。对来自三个来源（A、B和C）的玉米粗粒进行了类似的皮肤刺激性评估，在24小时时，所有样品在擦伤和未擦伤的测试部位均产生红斑和水肿。在72小时时，约一半的兔子出现焦痂，并持续到第7天实验结束。皮肤标本的组织学检查显示，所有三个玉米粗粒样品均引起了表皮炎。此外，接触来自两个来源（A和B）玉米粗粒的兔子，出现了中度局灶性至重度弥漫性化脓性坏死性毛囊炎和皮炎。在接受来自A和B来源玉米粗粒处理的兔子的毛囊微脓疱中，可见与毛霉目物种相符的大型管状分支无隔菌丝。应用于每个来源玉米粗粒的真菌培养技术显示，从A和C来源的样品中分离出了根霉属的一种潜在病原体，而B来源的样品中未分离出。皮肤刺激性试验包括将覆盖有纱布的测试材料应用于擦伤和未擦伤的皮肤。在纱布上放置橡胶挡圈并用胶带固定。暴露24小时后，去除所有绷带和可见的测试材料。去除后立即评估皮肤刺激性，然后定期评估，直至第7天实验结束。
