Harmsen A G, Muggenburg B A, Snipes M B, Bice D E
Science. 1985 Dec 13;230(4731):1277-80. doi: 10.1126/science.4071052.
Red fluorescent and green fluorescent microspheres were instilled into separate but adjacent areas of dog lung lobes. After 7 days, the tracheobronchial lymph nodes that drained both of the instilled areas contained many macrophages with all red or all green microspheres but rarely both. This indicates that the particles did not translocate passively and that lung macrophages phagocytized the microspheres in the lung and carried them to the tracheobronchial lymph nodes. In addition, two populations of pulmonary alveolar macrophages (PAM's), one that had phagocytized red microspheres in vivo and one that had phagocytized green microspheres, were lavaged from the lungs of dogs, mixed into one population, and instilled back into a previously unexposed lung lobe of the same dogs. As in the first experiment, the tracheobronchial lymph nodes that drained the instilled area contained numerous macrophages with either all red or all green microspheres. This suggested that the instilled PAM's had migrated to the tracheobronchial lymph nodes. Thus, lung macrophages, including PAM's probably play a critical role in the induction of lung immunity and in protection from disease by determining particle translocation.
将红色荧光微球和绿色荧光微球分别注入犬肺叶相邻的不同区域。7天后，引流这两个注入区域的气管支气管淋巴结含有许多巨噬细胞，这些巨噬细胞内的微球要么全是红色，要么全是绿色，很少同时含有两种颜色的微球。这表明颗粒不会被动转运，肺巨噬细胞会在肺内吞噬微球并将它们运送至气管支气管淋巴结。此外，从犬肺中冲洗出两类肺泡巨噬细胞（PAM），一类在体内吞噬了红色微球，另一类吞噬了绿色微球，将它们混合后再注入同一只犬先前未暴露的肺叶。与第一个实验一样，引流注入区域的气管支气管淋巴结含有大量巨噬细胞，其微球要么全是红色，要么全是绿色。这表明注入的PAM迁移到了气管支气管淋巴结。因此，包括PAM在内的肺巨噬细胞可能通过决定颗粒转运，在诱导肺免疫和预防疾病中发挥关键作用。
