Zhang Lu, Bai Jun, Li Long, Jia Yanqing, Qiu Xinxin, Luo Yan, Zhou Dong, Zhang Zhencang
Department of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, China.
Shaanxi Engineering Research Center of the Prevention and Control for Animal Disease, Yangling Vocational & Technical College, Yangling 712100, China.
Vet Sci. 2025 Jun 20;12(7):605. doi: 10.3390/vetsci12070605.
Brucellosis, caused by species, remains a significant zoonotic disease affecting both human and animal health worldwide. Among the outer membrane proteins (Omps) of , Omp16 has emerged as a key immunogenic target with potential applications in vaccine development and diagnostics. Omp16, a lipidated peptidoglycan-associated lipoprotein, stimulates a strong proinflammatory response and is essential for maintaining the bacterial outer membrane integrity and facilitating host cell invasion. This review examines the immunogenic properties of Omp16, its role in pathogenesis, and its potential as a candidate for vaccine development. We discuss how Omp16-based vaccines, including recombinant proteins, outer membrane vesicles, and viral vector vaccines, have shown promise in providing protection against infections in animal models. Additionally, Omp16's utility in diagnostic applications, particularly in enzyme-linked immunosorbent assays (ELISA), offers a reliable method for detecting brucellosis in both humans and animals. Overall, Omp16 represents a crucial antigen with significant potential for advancing both the diagnosis and prevention of brucellosis, offering insights into the next generation of brucellosis vaccines and diagnostic tools.
布鲁氏菌病由布鲁氏菌属物种引起，仍然是一种严重的人畜共患病，在全球范围内影响着人类和动物的健康。在布鲁氏菌的外膜蛋白（Omps）中，Omp16已成为关键的免疫原性靶点，在疫苗开发和诊断方面具有潜在应用价值。Omp16是一种脂化的肽聚糖相关脂蛋白，可刺激强烈的促炎反应，对于维持细菌外膜完整性和促进宿主细胞侵袭至关重要。本综述探讨了Omp16的免疫原性特性、其在布鲁氏菌病发病机制中的作用以及作为疫苗开发候选物的潜力。我们讨论了基于Omp16的疫苗，包括重组蛋白、外膜囊泡和病毒载体疫苗，在动物模型中针对布鲁氏菌感染提供保护方面已显示出前景。此外，Omp16在诊断应用中的效用，特别是在酶联免疫吸附测定（ELISA）中，为检测人和动物的布鲁氏菌病提供了一种可靠的方法。总体而言，Omp16是一种关键抗原，在推进布鲁氏菌病的诊断和预防方面具有巨大潜力，为下一代布鲁氏菌病疫苗和诊断工具提供了思路。
