Cai Ziwen, Zhang Wenjing, Wang Yun, Yang Zhaoying, Lei Xiaolei, Shi Xiaomin, Du Mengze, Liu Xiaoye
Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, No. 7 Beinong Road, Changping, Beijing 102206, China.
Vet Sci. 2025 Jun 23;12(7):611. doi: 10.3390/vetsci12070611.
The intensive farming of () for its medicinal and economic value has led to an increased incidence of bacterial skin infections, commonly treated with antibiotics. However, rising antibiotic resistance has reduced the effectiveness of these treatments, highlighting an urgent need for alternative therapeutic approaches. In this study, we isolated and identified pathogenic bacteria ( and ) from affected by skin ulcer disease at a turtle farm in Beijing. Antibiotic resistance was evaluated using the checkerboard microdilution method and the in vitro antibacterial efficacy of a florfenicol-doxycycline combination therapy was assessed. A total of 30 bacterial isolates were obtained, primarily and species. Among these, 83.3% of and 57.14% of isolates demonstrated multidrug-resistance. The florfenicol-doxycycline combination yielded lower fractional inhibitory concentration (FIC) values, indicating synergistic or additive effects. Animal experiments have shown that florfenicol in combination with doxycycline can achieve better therapeutic effects with less drug. These findings support that combination antibiotic therapies help in managing skin ulcer disease in and offer valuable insights for clinical applications.
由于其药用和经济价值而对（）进行集约化养殖，导致细菌性皮肤感染的发病率上升，通常使用抗生素进行治疗。然而，抗生素耐药性的增加降低了这些治疗方法的有效性，凸显了对替代治疗方法的迫切需求。在本研究中，我们从北京一家龟场患有皮肤溃疡病的（）中分离并鉴定出病原菌（和）。使用棋盘微量稀释法评估抗生素耐药性，并评估氟苯尼考-强力霉素联合疗法的体外抗菌效果。共获得30株细菌分离株，主要为（）和（）菌种。其中，83.3%的（）和57.14%的（）分离株表现出多重耐药性。氟苯尼考-强力霉素联合用药产生了较低的最低抑菌浓度（FIC）值，表明具有协同或相加作用。动物实验表明，氟苯尼考与强力霉素联合使用可以用更少的药物达到更好的治疗效果。这些发现支持联合抗生素疗法有助于治疗（）的皮肤溃疡病，并为临床应用提供了有价值的见解。
