Feng Erkai, Ye Zihan, Yan Manping, Zhou Yaxi, Wu Danni, Cheng Shipeng, Cheng Yuening
Key Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Science, Chinese Academy of Agriculture Science, Changchun 130011, China.
Vet Sci. 2025 Jul 16;12(7):668. doi: 10.3390/vetsci12070668.
To genetically characterise an epidemic isolate of feline panleukopenia virus (FPLV) harbouring the Ala91Ser mutation in China, a clinical strain (accession number: OR921195.1), named FPLV-CC19-02, was isolated from a PCR-positive faecal swab sample. Phylogenetic analysis revealed that it is far removed from all current commercial vaccine strains and differs from the FPLV prototype strain Cu-4 (M38246.1), specifically the vaccine strain of Fel-O-Vax PCT, at positions 91 (Ala91Ser) and 101 (Ile101Thr) within the VP2 protein. This virus can induce the typical cytopathic effect seen in parvovirus infection in feline kidney cells, resulting in severe clinical symptoms in cats, including haematochezia and hyperthermia. Furthermore, infected cats died of virus infection within 5-10 days post-infection (dpi) (100% morbidity and 83% mortality), indicating that FPLV-CC19-02 is a strain with increased virulence. Additionally, it demonstrated good immunogenicity in cats. Overall, these findings may help us to better understand the molecular prevalence of feline panleukopenia virus in cats and provide valuable basic data for the development of effective, locally adapted feline panleukopenia virus vaccines in China.
为从基因层面鉴定中国一株携带Ala91Ser突变的猫泛白细胞减少症病毒（FPLV）流行毒株，从一份PCR检测呈阳性的粪便拭子样本中分离出一株临床毒株（登录号：OR921195.1），命名为FPLV-CC19-02。系统发育分析显示，它与所有当前的商业疫苗毒株差异很大，并且在VP2蛋白的第91位（Ala91Ser）和第101位（Ile101Thr）与FPLV原型毒株Cu-4（M38246.1）不同，特别是Fel-O-Vax PCT疫苗毒株。该病毒可在猫肾细胞中诱导细小病毒感染中常见的典型细胞病变效应，导致猫出现严重的临床症状，包括血便和高热。此外，感染的猫在感染后5-10天内死于病毒感染（发病率100%，死亡率83%），表明FPLV-CC19-02是一种毒力增强的毒株。此外，它在猫身上表现出良好的免疫原性。总体而言，这些发现可能有助于我们更好地了解猫泛白细胞减少症病毒在猫中的分子流行情况，并为在中国开发有效的、适用于当地的猫泛白细胞减少症病毒疫苗提供有价值的基础数据。
