Okamoto Naoki, Sato Yorino, Kawagoe Yuta, Kawamura Kazuhiro
Department of Food and Reproductive Function, Juntendo University Faculty of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.
Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan.
Aging (Albany NY). 2025 Jul 23;17(7):1762-1783. doi: 10.18632/aging.206287.
Aging is associated with a decline in oocyte quality, which reduces the likelihood of successful pregnancy and childbirth. Recent research has focused on dietary supplements to improve oocyte quality, with particular interest in resveratrol, a natural polyphenol. Pterostilbene, a dimethoxylated analog of resveratrol, has demonstrated greater physiological activity. This study investigates the effects of pterostilbene on reproductive outcomes in mice. Short-term (1-week) ingestion of pterostilbene by aged mice resulted in an increased implantation rate. In middle-aged mice, long-term (22-week) ingestion enhanced implantation and live birth rates, increased the number of ovulated oocytes, and reduced miscarriage rates, correlating with serum pterostilbene levels. Importantly, pterostilbene ingestion did not affect the estrous cycle or body weight, and offspring showed no health or reproductive abnormalities. At the cellular level, pterostilbene treatment improved mitochondrial membrane potential and ATP levels in oocytes, though it did not alter mitochondrial DNA copy number. Unlike resveratrol, pterostilbene did not inhibit decidualization in endometrial stromal cells. These findings suggest that pterostilbene effectively restores oocyte quality in aged mice and prevents age-related oocyte deterioration in middle-aged mice without adverse effects on the animals or their progeny.
衰老与卵母细胞质量下降有关，这降低了成功怀孕和分娩的可能性。最近的研究集中在膳食补充剂以提高卵母细胞质量，尤其关注白藜芦醇这种天然多酚。紫檀芪是白藜芦醇的二甲氧基化类似物，已显示出更强的生理活性。本研究调查了紫檀芪对小鼠生殖结局的影响。老年小鼠短期（1周）摄入紫檀芪导致着床率增加。中年小鼠长期（22周）摄入则提高了着床率和活产率，增加了排卵的卵母细胞数量，并降低了流产率，这与血清紫檀芪水平相关。重要的是，摄入紫檀芪不影响发情周期或体重，且后代未出现健康或生殖异常。在细胞水平上，紫檀芪处理改善了卵母细胞的线粒体膜电位和ATP水平，不过并未改变线粒体DNA拷贝数。与白藜芦醇不同，紫檀芪不抑制子宫内膜基质细胞的蜕膜化。这些发现表明，紫檀芪可有效恢复老年小鼠的卵母细胞质量，并防止中年小鼠出现与年龄相关的卵母细胞退化，且对动物及其后代无不良影响。
