Srivatanakul P, Viyanant V, Kurathong S, Tiwawech D
Southeast Asian J Trop Med Public Health. 1985 Jun;16(2):234-9.
Antibodies to O. viverrini in the sera of people from endemic and non-endemic areas were investigated using indirect ELISA technique. For the patients from the endemic area, 92.8% who passed eggs in the stool were found to be positive for O. viverrini antibody. In addition, 46.5% of the people who did not pass eggs in the stool were also found to have low titer of O. viverrini antibody. On the other hand only 2.4% of the people from the non-endemic area with other intestinal parasite infections were found to have O. viverrini antibody in their sera. It was concluded that positive reaction of O. viverrini antibody is not cause by cross-reaction with other parasites but low liter of antibody is probably due to low-level or past infection. There is a positive correlation between the titer of O. viverrini antibody and intensity of infection as indicated by number of eggs excreted per milligram of feces. Patients with a few O. viverrini eggs in feces, but biopsy-proved-cholangiocarcinoma had very high titer of antibody.
采用间接酶联免疫吸附测定（ELISA）技术，对来自流行区和非流行区人群血清中的华支睾吸虫抗体进行了检测。对于来自流行区的患者，粪便中排出虫卵的患者中有92.8%被检测出华支睾吸虫抗体呈阳性。此外，粪便中未排出虫卵的人群中，有46.5%也被检测出华支睾吸虫抗体滴度较低。另一方面，在非流行区患有其他肠道寄生虫感染的人群中，只有2.4%的人血清中检测出华支睾吸虫抗体。研究得出结论，华支睾吸虫抗体呈阳性反应并非由与其他寄生虫的交叉反应引起，而抗体滴度较低可能是由于感染程度较低或既往感染所致。华支睾吸虫抗体滴度与感染强度之间存在正相关，感染强度以每毫克粪便中排出的虫卵数量表示。粪便中华支睾吸虫虫卵数量较少但经活检证实患有胆管癌的患者，其抗体滴度非常高。
