Tanvanich S, Doungchawee G, Boonpucknavig S, Thamavit W
Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Clin Exp Immunol. 1988 Dec;74(3):355-8.
The protein compositions of homogenized metacercaria and adult Opisthorchis viverrini were characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The two different stages of O. viverrini parasite contained several protein components with very similar mobilities on SDS-PAGE gel. The reaction in immunoblots of sera from patients with opisthorchiasis demonstrated antibodies against heterogeneous protein components of metacercariae and adult O. viverrini parasites. Only the components of metacercariae with molecular weights of 190-200 kD, 132 kD and 107 kD reacted specifically with opisthorchiasis sera. This characteristic reaction pattern was not observed in any control sera of normal subjects or those infected with other parasites. Identical reactions were however also recognized in the sera of experimental infected hamsters with metacercariae, but did not occur with adult worm as the antigen for the immunoblotting reaction. This may indicate that these metacercariae-associated protein components have potential diagnostic value for opisthorchiasis.
采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）对肝片吸虫后尾蚴和成虫匀浆的蛋白质组成进行了表征。肝片吸虫寄生虫的这两个不同阶段在SDS-PAGE凝胶上含有几种迁移率非常相似的蛋白质成分。华支睾吸虫病患者血清免疫印迹反应显示，其针对后尾蚴和成虫肝片吸虫寄生虫的异质蛋白质成分产生了抗体。只有分子量为190 - 200 kD、132 kD和107 kD的后尾蚴成分与华支睾吸虫病血清发生特异性反应。在正常受试者或感染其他寄生虫的对照血清中未观察到这种特征性反应模式。然而，在用后尾蚴进行实验感染的仓鼠血清中也观察到了相同的反应，但以成虫作为免疫印迹反应的抗原时则未出现这种反应。这可能表明这些与后尾蚴相关的蛋白质成分对华支睾吸虫病具有潜在的诊断价值。
