Su Weikang, Zhong Zhiling, Li Huazhi, Meng Lingjia, Zhong Xinqiang
Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou, 510632, China.
Department of Gastroenterology, The Second Hospital of Dalian Medical University, Dalian, 116000, China.
Hereditas. 2025 Jul 26;162(1):144. doi: 10.1186/s41065-025-00511-7.
Gastric cancer (GC) remains a global health challenge due to its high morbidity, late-stage diagnosis, and limited therapeutic options. long non-coding RNA PANDAR has been implicated in tumorigenesis across multiple cancers, yet its role in GC pathogenesis and diagnostic utility is still unclear. The purpose of this study is to elucidate the diagnostic value of serum PANDAR and its regulatory mechanisms in GC progression.
lncRNA PANDAR levels were quantified in serum from 112 GC patients and 98 benign controls using RT-qPCR. The diagnostic value of lncRNA PANDAR was analyzed by ROC curve. Functional experiments (CCK-8, Transwell assays) and dual-luciferase reporter assays were conducted in HGC-27 and BGC-823 cells to explore the function of PANDAR in cell proliferation, migration, and invasion. The interaction between PANDAR and miR-637 was validated via bioinformatics prediction and co-inhibition assay.
Serum PANDAR was significantly upregulated in GC patients (P < 0.0001) and exhibited high diagnostic accuracy (AUC = 0.913). Elevated PANDAR associated with advanced TNM stage (P = 0.047), lymph node metastasis (P = 0.023), and digestive system history (P = 0.035). PANDAR knockdown suppressed GC cell proliferation, migration, and invasion, effects reversed by co-inhibition of miR-637.
lncRNA PANDAR is a promising non-invasive diagnostic indicator of GC, and may serve as a biomarker for GC diagnosis and therapy. lncRNA PANDAR regulates the progression of GC through sponge miR-637.
胃癌（GC）因其高发病率、晚期诊断和有限的治疗选择，仍然是一项全球性的健康挑战。长链非编码RNA PANDAR已被证明与多种癌症的肿瘤发生有关，但其在胃癌发病机制和诊断效用中的作用仍不清楚。本研究的目的是阐明血清PANDAR在胃癌进展中的诊断价值及其调控机制。
使用RT-qPCR对112例胃癌患者和98例良性对照者血清中的lncRNA PANDAR水平进行定量。通过ROC曲线分析lncRNA PANDAR的诊断价值。在HGC-27和BGC-823细胞中进行功能实验（CCK-8、Transwell实验）和双荧光素酶报告基因实验，以探讨PANDAR在细胞增殖、迁移和侵袭中的功能。通过生物信息学预测和共抑制实验验证PANDAR与miR-637之间的相互作用。
胃癌患者血清PANDAR显著上调（P < 0.0001），并表现出较高的诊断准确性（AUC = 0.913）。PANDAR升高与晚期TNM分期（P = 0.047）、淋巴结转移（P = 0.023）和消化系统病史（P = 0.035）相关。PANDAR敲低抑制了胃癌细胞的增殖、迁移和侵袭，miR-637共抑制可逆转这些作用。
lncRNA PANDAR是一种有前景的胃癌非侵入性诊断指标，可能作为胃癌诊断和治疗的生物标志物。lncRNA PANDAR通过海绵化miR-637调节胃癌的进展。
