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通过聚丙烯酰胺凝胶等电聚焦和免疫印迹分析抗凝血酶III的微观不均一性

Analysis of antithrombin III microheterogeneity by isoelectric focusing in polyacrylamide gels and immunoblotting.

作者信息

Daly M, Hallinan F

出版信息

Thromb Res. 1985 Oct 15;40(2):207-14. doi: 10.1016/0049-3848(85)90331-7.

DOI:10.1016/0049-3848(85)90331-7
PMID:4071470
Abstract

Microheterogeneity of antithrombin III (ATIII) was examined in pooled normal human plasma and serum by IEF/PA and immunoblotting techniques. A pattern consisting of 8 bands varying in isoelectric point from 4.7 to 5.2 was observed in citrated plasma following IEF in a pH range of 4-6. A similar pattern was observed in all 149 healthy adults examined. The reactivity of ATIII with heparin or, thrombin was investigated using this technique. Characteristic shifts of the banding pattern to the anode or, cathode respectively were observed.

摘要

采用等电聚焦/聚丙烯酰胺凝胶电泳（IEF/PA）和免疫印迹技术检测了正常人混合血浆和血清中抗凝血酶III（ATIII）的微观异质性。在pH值为4 - 6的范围内进行IEF后，在枸橼酸盐血浆中观察到由8条等电点在4.7至5.2之间变化的条带组成的图谱。在所有检测的149名健康成年人中均观察到类似的图谱。使用该技术研究了ATIII与肝素或凝血酶的反应性。分别观察到条带图谱向阳极或阴极的特征性移动。

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