• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种用于即时检测和活细胞检测D-2-羟基戊二酸的基因编码生物传感器。

A genetically encoded biosensor for point-of-care and live-cell detection of D-2-hydroxyglutarate.

作者信息

Liu Yidong, Kang Zhaoqi, Xu Rong, Hou Shuang, Gong Lingru, Guo Leilei, Gao Kaiyu, Xu Xianzhi, Tan Xiaoxu, Xiao Dan, Zhang Wen, Wang Gang, Ou Shaowu, Hu Jinqu, Liu Jiyuan, Lü Chuanjuan, Ma Cuiqing, Xu Ping, Gao Chao

机构信息

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, People's Republic of China.

Institute of Medical Sciences, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People's Republic of China.

出版信息

Nat Commun. 2025 Jul 26;16(1):6913. doi: 10.1038/s41467-025-62225-8.

DOI:10.1038/s41467-025-62225-8
PMID:40715112
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12297413/
Abstract

D-2-Hydroxyglutarate (D-2-HG) is a functional endogenous metabolite in various domains of life. Its abnormal accumulation promotes human tumorigenesis. Convenient D-2-HG testing for diagnosis and prognosis of D-2-HG-related diseases remains technically challenging, and there is no analytical method to directly detect D-2-HG in living cells. Here, we identify a D-2-HG-specific transcriptional activator, HgcR, and develop a d-2-HG sensor (DHOR) using HgcR as a sensing moiety. Then, we build a portable device adaptive with DHOR for rapid and low-cost point-of-care D-2-HG testing in serum, urine, and glioma tissue samples. DHOR also allow spatiotemporal resolution of D-2-HG in living bacteria and human cells. We use DHOR to identify D-2-HG transporters from Escherichia coli and human solute carrier 22 family. Overall, DHOR provides a powerful and versatile tool for in vitro and live-cell detection of D-2-HG, offering the opportunity to deepen our understanding about physiological and pathogenetic roles of D-2-HG.

摘要

D-2-羟基戊二酸（D-2-HG）是生命各个领域中的一种功能性内源性代谢物。其异常积累会促进人类肿瘤发生。对D-2-HG相关疾病进行诊断和预后的便捷D-2-HG检测在技术上仍然具有挑战性，并且尚无直接检测活细胞中D-2-HG的分析方法。在此，我们鉴定出一种D-2-HG特异性转录激活因子HgcR，并开发了一种以HgcR作为传感部分的D-2-HG传感器（DHOR）。然后，我们构建了一种与DHOR适配的便携式设备，用于在血清、尿液和胶质瘤组织样本中进行快速且低成本的即时D-2-HG检测。DHOR还能对活细菌和人类细胞中的D-2-HG进行时空分辨。我们利用DHOR从大肠杆菌和人类溶质载体22家族中鉴定出D-2-HG转运蛋白。总体而言，DHOR为体外和活细胞检测D-2-HG提供了一种强大且通用的工具，为加深我们对D-2-HG的生理和致病作用的理解提供了契机。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/3b0dde5bae49/41467_2025_62225_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/39bdcc7b0d1b/41467_2025_62225_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/e3f0118bdb1e/41467_2025_62225_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/1ca73c8d7fc2/41467_2025_62225_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/a758938ee29c/41467_2025_62225_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/170b8df51321/41467_2025_62225_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/3b0dde5bae49/41467_2025_62225_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/39bdcc7b0d1b/41467_2025_62225_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/e3f0118bdb1e/41467_2025_62225_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/1ca73c8d7fc2/41467_2025_62225_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/a758938ee29c/41467_2025_62225_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/170b8df51321/41467_2025_62225_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6f/12297413/3b0dde5bae49/41467_2025_62225_Fig6_HTML.jpg

相似文献

1
A genetically encoded biosensor for point-of-care and live-cell detection of D-2-hydroxyglutarate.一种用于即时检测和活细胞检测D-2-羟基戊二酸的基因编码生物传感器。
Nat Commun. 2025 Jul 26;16(1):6913. doi: 10.1038/s41467-025-62225-8.
2
Diagnostic test accuracy and cost-effectiveness of tests for codeletion of chromosomal arms 1p and 19q in people with glioma.染色体臂 1p 和 19q 缺失的检测在胶质瘤患者中的诊断准确性和成本效益。
Cochrane Database Syst Rev. 2022 Mar 2;3(3):CD013387. doi: 10.1002/14651858.CD013387.pub2.
3
Interventions for fertility preservation in women with cancer undergoing chemotherapy.对接受化疗的癌症女性进行生育力保存的干预措施。
Cochrane Database Syst Rev. 2025 Jun 19;6:CD012891. doi: 10.1002/14651858.CD012891.pub2.
4
Falls prevention interventions for community-dwelling older adults: systematic review and meta-analysis of benefits, harms, and patient values and preferences.社区居住的老年人跌倒预防干预措施：系统评价和荟萃分析的益处、危害以及患者的价值观和偏好。
Syst Rev. 2024 Nov 26;13(1):289. doi: 10.1186/s13643-024-02681-3.
5
Bioengineered nerve conduits and wraps for peripheral nerve repair of the upper limb.生物工程神经导管和套用于上肢周围神经修复。
Cochrane Database Syst Rev. 2022 Dec 7;12(12):CD012574. doi: 10.1002/14651858.CD012574.pub2.
6
Point-of-care tests for urinary tract infections to reduce antimicrobial resistance: a systematic review and conceptual economic model.用于减少抗菌药物耐药性的尿路感染即时检测：一项系统评价和概念性经济模型
Health Technol Assess. 2024 Nov;28(77):1-109. doi: 10.3310/PTMV8524.
7
Perceptions and experiences of the prevention, detection, and management of postpartum haemorrhage: a qualitative evidence synthesis.预防、检测和管理产后出血的认知和经验：定性证据综合。
Cochrane Database Syst Rev. 2023 Nov 27;11(11):CD013795. doi: 10.1002/14651858.CD013795.pub2.
8
Carbon dioxide detection for diagnosis of inadvertent respiratory tract placement of enterogastric tubes in children.用于诊断儿童肠胃管意外置入呼吸道的二氧化碳检测
Cochrane Database Syst Rev. 2025 Feb 19;2(2):CD011196. doi: 10.1002/14651858.CD011196.pub2.
9
In vitro maturation in subfertile women with polycystic ovarian syndrome undergoing assisted reproduction.多囊卵巢综合征不孕妇女在辅助生殖过程中的体外成熟。
Cochrane Database Syst Rev. 2025 Feb 6;2(2):CD006606. doi: 10.1002/14651858.CD006606.pub5.
10
Conservative, physical and surgical interventions for managing faecal incontinence and constipation in adults with central neurological diseases.保守治疗、物理治疗和手术干预用于治疗伴有中枢神经系统疾病的成年人的粪便失禁和便秘。
Cochrane Database Syst Rev. 2024 Oct 29;10(10):CD002115. doi: 10.1002/14651858.CD002115.pub6.

本文引用的文献

1
A genetically encoded fluorescent sensor enables sensitive and specific detection of IDH mutant associated oncometabolite D-2-hydroxyglutarate.一种基因编码的荧光传感器能够灵敏且特异地检测异柠檬酸脱氢酶（IDH）突变相关的致癌代谢物D-2-羟基戊二酸。
BMC Cancer. 2025 Mar 14;25(1):473. doi: 10.1186/s12885-025-13877-8.
2
Lighting up arginine metabolism reveals its functional diversity in physiology and pathology.点亮精氨酸代谢揭示其在生理和病理中的功能多样性。
Cell Metab. 2025 Jan 7;37(1):291-304.e9. doi: 10.1016/j.cmet.2024.09.011. Epub 2024 Oct 15.
3
An Ultrasensitive Biosensor for Probing Subcellular Distribution and Mitochondrial Transport of l-2-Hydroxyglutarate.
一种用于探测 l-2-羟戊二酸亚细胞分布和线粒体转运的超灵敏生物传感器。
Adv Sci (Weinh). 2024 Sep;11(35):e2404119. doi: 10.1002/advs.202404119. Epub 2024 Jul 15.
4
Rapid detection of IDH mutations in gliomas by intraoperative mass spectrometry.通过术中质谱法快速检测脑胶质瘤中的 IDH 突变。
Proc Natl Acad Sci U S A. 2024 Jun 4;121(23):e2318843121. doi: 10.1073/pnas.2318843121. Epub 2024 May 28.
5
Accurate structure prediction of biomolecular interactions with AlphaFold 3.利用 AlphaFold 3 进行生物分子相互作用的精确结构预测。
Nature. 2024 Jun;630(8016):493-500. doi: 10.1038/s41586-024-07487-w. Epub 2024 May 8.
6
A biosensor for D-2-hydroxyglutarate in frozen sections and intraoperative assessment of IDH mutation status.一种用于冰冻切片中 D-2-羟戊二酸的生物传感器及其在 IDH 突变状态术中评估中的应用。
Biosens Bioelectron. 2024 Mar 1;247:115921. doi: 10.1016/j.bios.2023.115921. Epub 2023 Dec 12.
7
UCSF ChimeraX: Tools for structure building and analysis.UCSF ChimeraX：结构构建和分析工具。
Protein Sci. 2023 Nov;32(11):e4792. doi: 10.1002/pro.4792.
8
Development of a 2-hydroxyglutarate production system by Corynebacterium glutamicum.利用谷氨酸棒杆菌开发 2-羟戊二酸生产系统。
Appl Microbiol Biotechnol. 2023 Oct;107(19):5987-5997. doi: 10.1007/s00253-023-12716-9. Epub 2023 Aug 9.
9
Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease.超敏传感器揭示了生理学和疾病中乳酸代谢的时空图谱。
Cell Metab. 2023 Jan 3;35(1):200-211.e9. doi: 10.1016/j.cmet.2022.10.002. Epub 2022 Oct 28.
10
Oncometabolite d-2HG alters T cell metabolism to impair CD8 T cell function.代谢物 d-2HG 改变 T 细胞代谢，从而损害 CD8+T 细胞的功能。
Science. 2022 Sep 30;377(6614):1519-1529. doi: 10.1126/science.abj5104. Epub 2022 Sep 29.