Johnson E C, Young M K, Stacy P A, Beatty C H
Clin Chim Acta. 1979 Oct 15;98(1-2):77-85. doi: 10.1016/0009-8981(79)90168-2.
We compared glucose metabolism by erythrocytes from patients with Duchenne muscular dystrophy and by erythrocytes from control individuals. There was a significant decrease in the rate of lactate production at pH 7.2 in the dystrophic group. When the pH of the incubation medium was changed to 8.0, we found that the increase in the rate of lactate production for the dystrophic group was significantly larger. We measured the concentrations of key glycolytic metabolites and adenine nucleotides and determined the values of the energy charge during these incubations. We also determined the concentrations of polyol pathway intermediates, the activities of the oxidative portion of the pentose phosphate pathway and the activities and kinetics of phosphofructokinase from both cell groups. There were no significant differences between groups for any of these variables.
我们比较了杜氏肌营养不良症患者红细胞与对照个体红细胞的葡萄糖代谢情况。在营养不良组中，pH 7.2时乳酸生成速率显著降低。当将孵育培养基的pH值变为8.0时，我们发现营养不良组乳酸生成速率的增加显著更大。我们测量了关键糖酵解代谢物和腺嘌呤核苷酸的浓度，并确定了这些孵育过程中的能荷值。我们还测定了两组细胞中多元醇途径中间体的浓度、磷酸戊糖途径氧化部分的活性以及磷酸果糖激酶的活性和动力学。这些变量在两组之间均无显著差异。
