Wang Yu, Xue Jiahao, Qiu Yue, Zhang Xiaopeng, Wang Dan, Chen Xiaoming, Gao Peng, Du Liquan, Xie Fei, Xia Xiaojun, Liu Qiang, Zeng Yu
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, China.
Irradiation Preservation Technology Key Laboratory of Sichuan Province, Sichuan Institute of Atomic Energy, Chengdu, China.
World J Microbiol Biotechnol. 2025 Jul 28;41(8):279. doi: 10.1007/s11274-025-04493-6.
The purpose of this study is to investigate the feasibility of utilizing high-energy electron beam irradiation for insecticidal purposes in Strong-flavor Baijiu Daqu (SBD) and to ascertain the irradiation threshold tolerable by SBD. According to the standard plate counting method, Illumina-MiSeq sequencing method and relevant industry testing standards, the effects of different irradiation doses (0-3.55 kGy) on the microbiome and Physicochemical properties of SBD were determined. Following irradiation, a reduction in the microbial population of SBD was observed, commensurate with the dose administered. However, subsequent to a 3-month storage period, SBD samples subjected to irradiation doses of 1.40 kGy or lower exhibited negligible disparities in physicochemical properties, microbial population, and microbial community structure when compared to non-irradiated counterparts. Conversely, irradiation doses exceeding 1.40 kGy resulted in marginal variations in physicochemical properties while notable discrepancies were noted in microbial abundance and community composition. Principal Coordinate Analysis (PCoA) revealed robust positive correlations between specific microbial taxa (e.g., Acetobacter and liquefaction, Saccharopolyspora and liquefaction, Streptomyces and saccharification, Thermoascus and acidity, Byssochlamys and moisture). Assessment of the efficacy of radiation doses ranging from 0 to 1.40 kGy in pest eradication indicated that a minimum irradiation dose of 0.27 kGy is imperative to prevent pest infestation during a storage duration of up to 120 days. In conclusion, this study proposes an optimal irradiation dosage range for insecticidal purposes in SBD, falling between 0.27 and 1.40 kGy. Additionally, it underscores the potential of high-energy electron beam irradiation as a viable method for quality assurance.
本研究旨在探讨利用高能电子束辐照进行浓香型白酒大曲（SBD）杀虫的可行性，并确定SBD可耐受的辐照阈值。根据标准平板计数法、Illumina-MiSeq测序法及相关行业检测标准，测定了不同辐照剂量（0 - 3.55 kGy）对SBD微生物群落和理化性质的影响。辐照后，观察到SBD微生物数量减少，且与辐照剂量成正比。然而，在3个月的储存期后，与未辐照的对照相比，接受1.40 kGy及以下辐照剂量的SBD样品在理化性质、微生物数量和微生物群落结构方面的差异可忽略不计。相反，超过1.40 kGy的辐照剂量导致理化性质有微小变化，而微生物丰度和群落组成则出现显著差异。主坐标分析（PCoA）显示特定微生物类群（如醋酸杆菌与液化、糖多孢菌与液化、链霉菌与糖化、嗜热ascus与酸度、瓶霉与水分）之间存在强正相关。对0至1.40 kGy辐照剂量的杀虫效果评估表明，在长达120天储存期内，最低辐照剂量0.27 kGy对于防止害虫侵扰至关重要。总之，本研究提出了SBD杀虫的最佳辐照剂量范围，介于0.27至1.40 kGy之间。此外，强调了高能电子束辐照作为一种可行的质量保证方法的潜力。
