人血细胞中6-巯基嘌呤磷酸核糖基化的荧光测定法。
A fluorimetric assay of the phosphoribosylation of 6-mercaptopurine in human blood cells.
作者信息
Wong P C, Hey Y, Fowler J S
出版信息
Clin Chim Acta. 1979 Nov 15;99(1):43-51. doi: 10.1016/0009-8981(79)90138-4.
PMID:40720
Abstract
A new fluorimetric method to assay HGPRT (hypoxanthine-guanine phosphoribosyl transferase, EC 2.4.2.8) from human red cell lysates using 6MP (6-mercaptopurine) as substrate is described. This assay is compared with an existing spectrophotometric method using hypoxanthine as substrate. Precision, sensitivity limits and kinetic differences of these assays are reported.
摘要
描述了一种新的荧光测定法，该方法使用6-巯基嘌呤（6MP）作为底物，从人红细胞裂解物中测定次黄嘌呤-鸟嘌呤磷酸核糖基转移酶（HGPRT，EC 2.4.2.8）。将该测定法与现有的以次黄嘌呤为底物的分光光度法进行了比较。报告了这些测定法的精密度、灵敏度极限和动力学差异。
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