• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用基于FIJI的半自动工具进行高效的细胞质细胞定量分析。

Efficient cytoplasmic cell quantification using a semi-automated FIJI-based tool.

作者信息

Unger Lucas, Larsen Ulrik, Sharmine Shayla, Hossain Md Kaykobad, Legøy Thomas Aga, Vaudel Marc, Ghila Luiza, Chera Simona

机构信息

Mohn Research Center for Diabetes Precision Medicine, Department of Clinical Science, Faculty of Medicine, University of Bergen, Glasblokkene 1, Haukelandsbakken 15, 5009, Bergen, Norway.

Department of Clinical Science, Faculty of Medicine, University of Bergen, Postboks 7804, 5020, Bergen, Norway.

出版信息

Sci Rep. 2025 Jul 28;15(1):27509. doi: 10.1038/s41598-025-12144-x.

DOI:10.1038/s41598-025-12144-x
PMID:40721622
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12304148/
Abstract

Quantification of subcellular structures such as nuclei and cytoplasmic proteins using staining methods based on fluorescent dyes or fluorescently tagged antibodies are widely used in scientific research. Accurate high-throughput quantitation of these assays can be time consuming and challenging. Here, we present our FIJI based Semi-Automated counting Macro termed SAM, and we validate its accuracy against manual counting and other automated counting methods. By introducing this automated quantification tool, we aim to contribute to the ongoing efforts to enhance the reliability, efficiency, and standardization of immunostaining analysis in the field of diabetes research and beyond.

摘要

使用基于荧光染料或荧光标记抗体的染色方法对细胞核和细胞质蛋白等亚细胞结构进行定量分析在科学研究中被广泛应用。对这些检测进行准确的高通量定量可能既耗时又具有挑战性。在此，我们展示了基于FIJI的半自动计数宏程序，称为SAM，并将其准确性与手动计数和其他自动计数方法进行了验证。通过引入这种自动定量工具，我们旨在为正在进行的努力做出贡献，以提高糖尿病研究及其他领域免疫染色分析的可靠性、效率和标准化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/e348ecc1de3a/41598_2025_12144_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/da86c9182a34/41598_2025_12144_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/66272de95297/41598_2025_12144_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/aa47d2ef03b0/41598_2025_12144_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/be869ab65d75/41598_2025_12144_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/cce7cb659958/41598_2025_12144_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/ae1d2ee4df65/41598_2025_12144_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/c4ceac41c195/41598_2025_12144_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/e348ecc1de3a/41598_2025_12144_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/da86c9182a34/41598_2025_12144_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/66272de95297/41598_2025_12144_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/aa47d2ef03b0/41598_2025_12144_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/be869ab65d75/41598_2025_12144_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/cce7cb659958/41598_2025_12144_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/ae1d2ee4df65/41598_2025_12144_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/c4ceac41c195/41598_2025_12144_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/12304148/e348ecc1de3a/41598_2025_12144_Fig8_HTML.jpg

相似文献

1
Efficient cytoplasmic cell quantification using a semi-automated FIJI-based tool.使用基于FIJI的半自动工具进行高效的细胞质细胞定量分析。
Sci Rep. 2025 Jul 28;15(1):27509. doi: 10.1038/s41598-025-12144-x.
2
Automated Image-Based Wound Area Assessment in Outpatient Clinics Using Computer-Aided Methods: A Development and Validation Study.使用计算机辅助方法在门诊诊所进行基于图像的伤口面积自动评估：一项开发与验证研究。
Medicina (Kaunas). 2025 Jun 17;61(6):1099. doi: 10.3390/medicina61061099.
3
Leveraging a foundation model zoo for cell similarity search in oncological microscopy across devices.利用基础模型库进行跨设备肿瘤显微镜检查中的细胞相似性搜索。
Front Oncol. 2025 Jun 18;15:1480384. doi: 10.3389/fonc.2025.1480384. eCollection 2025.
4
Using Computer Vision Libraries to Streamline Nuclei Quantification.使用计算机视觉库简化细胞核定量分析。
J Vis Exp. 2025 Jun 6(220). doi: 10.3791/67945.
5
AI-based Hepatic Steatosis Detection and Integrated Hepatic Assessment from Cardiac CT Attenuation Scans Enhances All-cause Mortality Risk Stratification: A Multi-center Study.基于人工智能的心脏CT衰减扫描检测肝脂肪变性及综合肝脏评估可增强全因死亡风险分层：一项多中心研究
medRxiv. 2025 Jun 11:2025.06.09.25329157. doi: 10.1101/2025.06.09.25329157.
6
Automated devices for identifying peripheral arterial disease in people with leg ulceration: an evidence synthesis and cost-effectiveness analysis.用于识别下肢溃疡患者外周动脉疾病的自动化设备：证据综合和成本效益分析。
Health Technol Assess. 2024 Aug;28(37):1-158. doi: 10.3310/TWCG3912.
7
Semi-Automated Method for Analyzing Lymphatic Vessels From Immunofluorescence Images.用于从免疫荧光图像分析淋巴管的半自动方法。
Curr Protoc. 2025 Jun;5(6):e70160. doi: 10.1002/cpz1.70160.
8
A segment anything model-guided and match-based semi-supervised segmentation framework for medical imaging.一种用于医学成像的基于段式分割模型引导和匹配的半监督分割框架。
Med Phys. 2025 Mar 29. doi: 10.1002/mp.17785.
9
Cost-effectiveness of using prognostic information to select women with breast cancer for adjuvant systemic therapy.利用预后信息为乳腺癌患者选择辅助性全身治疗的成本效益
Health Technol Assess. 2006 Sep;10(34):iii-iv, ix-xi, 1-204. doi: 10.3310/hta10340.
10
Artificial Intelligence-Assisted Visualized Microspheres for Biochemical Analysis: From Encoding to Decoding.用于生化分析的人工智能辅助可视化微球：从编码到解码
Acc Chem Res. 2025 Aug 1. doi: 10.1021/acs.accounts.5c00396.

本文引用的文献

1
Mapping the initial effects of carcinogen-induced oncogenic transformation in the mouse bladder.描绘致癌物诱导的小鼠膀胱致癌转化的初始效应。
Exp Cell Res. 2025 Mar 15;446(2):114452. doi: 10.1016/j.yexcr.2025.114452. Epub 2025 Feb 21.
2
Moderate beta-cell ablation triggers synergic compensatory mechanisms even in the absence of overt metabolic disruption.中度β细胞消融会触发协同补偿机制，即使在没有明显代谢紊乱的情况下也是如此。
Commun Biol. 2024 Jul 9;7(1):833. doi: 10.1038/s42003-024-06527-5.
3
Molecular profiling of NOD mouse islets reveals a novel regulator of insulitis onset.
对 NOD 小鼠胰岛的分子谱分析揭示了一种新的胰岛炎发病调节剂。
Sci Rep. 2024 Jun 25;14(1):14669. doi: 10.1038/s41598-024-65454-x.
4
The age-dependent regulation of pancreatic islet landscape is fueled by a HNF1a-immune signaling loop.胰腺胰岛景观的年龄依赖性调节由一个HNF1a-免疫信号回路驱动。
Mech Ageing Dev. 2024 Aug;220:111951. doi: 10.1016/j.mad.2024.111951. Epub 2024 May 31.
5
Illuminating the complete ß-cell mass of the human pancreas- signifying a new view on the islets of Langerhans.阐明人类胰腺中的完整β细胞群——标志着对胰岛的新认识。
Nat Commun. 2024 Apr 18;15(1):3318. doi: 10.1038/s41467-024-47686-7.
6
Targeted Gene Silencing by Using GapmeRs in Differentiating Human-Induced Pluripotent Stem Cells (hiPSC) Toward Pancreatic Progenitors.利用 GapmeRs 在分化的人诱导多能干细胞 (hiPSC) 中靶向基因沉默以获得胰腺祖细胞。
Methods Mol Biol. 2024;2736:23-38. doi: 10.1007/7651_2023_498.
7
Evaluation of an Automated System for the Counting of Microbial Colonies.评价一种用于微生物菌落计数的自动化系统。
Microbiol Spectr. 2023 Aug 17;11(4):e0067323. doi: 10.1128/spectrum.00673-23. Epub 2023 Jul 3.
8
Differentiating functional human islet-like aggregates from pluripotent stem cells.从多能干细胞中分离功能性人胰岛样细胞团。
STAR Protoc. 2022 Dec 16;3(4):101711. doi: 10.1016/j.xpro.2022.101711. Epub 2022 Sep 21.
9
Reduced calcium levels and accumulation of abnormal insulin granules in stem cell models of HNF1A deficiency.HNF1A 缺陷的干细胞模型中钙水平降低和异常胰岛素颗粒的积累。
Commun Biol. 2022 Aug 2;5(1):779. doi: 10.1038/s42003-022-03696-z.
10
Determinants and dynamics of pancreatic islet architecture.胰岛结构的决定因素和动态变化。
Islets. 2022 Dec 31;14(1):82-100. doi: 10.1080/19382014.2022.2030649.