Wang Zishi, Yun Quanzheng, Hu Jinyuan, Wei Zhen, Feng Deri, Li Ning, Xu He, Fu Lihong, Wang Zhenlong, Li Shujin, Liu Fangru, Wang Yan, Cong Bin, Wang Baishi
School of Basic Medicine, Shenyang Key Laboratory for Phenomics, Liaoning Province Key Laboratory for Phenomics of Human Ethnic Specificity and Critical Illness (LPKL-PHESCI), Shenyang Medical College, Shenyang, 110034, China.
School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China.
Sci Rep. 2025 Jul 28;15(1):27406. doi: 10.1038/s41598-025-11942-7.
Benzylisoquinoline alkaloids (BIAs) are essential secondary metabolites produced by Papaver somniferum, widely recognized for their pharmaceutical importance. This study employs transcriptome sequencing and weighted gene co-expression network analysis (WGCNA) to investigate the spatiotemporal expression patterns and regulatory networks of BIA-related genes across developmental stages and organs. A total of 23 co-expression modules were identified, revealing stage- and organ-specific dynamics in BIA biosynthesis. Key genes such as TYDC, PPO, and GsSRK demonstrated distinct regulatory roles during flowering and fruit maturation. Functional enrichment analysis uncovered critical pathways and transcription factors involved in alkaloid production. These findings enhance our understanding of the molecular regulation of BIAs and provide valuable insights for improving alkaloid yield through metabolic engineering and molecular breeding strategies.
苄基异喹啉生物碱（BIAs）是罂粟产生的重要次生代谢产物，因其药学重要性而被广泛认可。本研究采用转录组测序和加权基因共表达网络分析（WGCNA）来研究BIA相关基因在不同发育阶段和器官中的时空表达模式及调控网络。共鉴定出23个共表达模块，揭示了BIA生物合成过程中特定阶段和器官的动态变化。TYDC、PPO和GsSRK等关键基因在开花和果实成熟过程中表现出不同的调控作用。功能富集分析揭示了参与生物碱生产的关键途径和转录因子。这些发现加深了我们对BIAs分子调控的理解，并为通过代谢工程和分子育种策略提高生物碱产量提供了有价值的见解。
