Gacem Sabrina, Mocé Eva, Gozalbo Carmen, Albuixech-Benetó Marta, Esteve Inés C, Martínez-Talaván Amparo, Silvestre Miguel A
Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, 46100 Valencia, Spain.
Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias, 12400 Castellón, Spain.
Biology (Basel). 2025 Jun 27;14(7):782. doi: 10.3390/biology14070782.
Artificial insemination in goats commonly relies on refrigerated semen doses, yet the optimal energetic substrate to support sperm metabolism remains unclear. This study aimed to evaluate the effects of different energetic substrates on goat buck sperm metabolism and motility when refrigerated at 17 °C. Semen from six Murciano-Granadina male goats were collected and diluted in PBS supplemented with 35 mM of either glucose, fructose, pyruvate, or lactate in the first experiment. In the second experiment, the effects of varying concentrations of pyruvate and/or glucose, NaCl supplementation, and the osmolarity on sperm quality parameters were assessed. Semen was stored at 17 °C for 48 h and evaluated for motility using the CASA system, as well as for viability, mitochondrial membrane potential, and mitochondrial ROS by flow cytometry. The results show that pyruvate and lactate extenders outperformed the others, preserving higher total motility, progressivity, and viability of spermatozoa over 48 h, even at a concentration lower than 35 mM, as in the case of pyruvate. In contrast, glucose had a detrimental effect on sperm quality, reducing viability and healthy population rates while increasing motility, especially at higher concentrations. NaCl supplementation and osmolarity had no significant effect on any of the sperm quality parameters. In conclusion, pyruvate maintains a higher quality and motility of sperm stored at 17 °C in PBS in comparison with a glucose-supplemented extender.
山羊人工授精通常依赖于冷冻精液剂量，但支持精子代谢的最佳能量底物仍不明确。本研究旨在评估在17℃冷藏时不同能量底物对山羊精液精子代谢和活力的影响。在第一个实验中，采集了6只穆尔西亚诺-格拉纳迪纳公山羊的精液，并将其稀释于补充有35 mM葡萄糖、果糖、丙酮酸或乳酸的磷酸盐缓冲液（PBS）中。在第二个实验中，评估了不同浓度的丙酮酸和/或葡萄糖、氯化钠补充以及渗透压对精子质量参数的影响。精液在17℃保存48小时，使用计算机辅助精子分析（CASA）系统评估活力，并通过流式细胞术评估存活率、线粒体膜电位和线粒体活性氧。结果表明，丙酮酸和乳酸稀释液的效果优于其他稀释液，在48小时内保持了更高的精子总活力、前进性和存活率，即使在低于35 mM的浓度下也是如此，如丙酮酸的情况。相比之下，葡萄糖对精子质量有不利影响，降低了存活率和健康群体率，同时增加了活力，尤其是在较高浓度下。补充氯化钠和渗透压对任何精子质量参数均无显著影响。总之，与补充葡萄糖的稀释液相比，丙酮酸能使在17℃保存在PBS中的精子保持更高的质量和活力。
