Liu Senyue, Li Qiang, Deng Yongqiang, Wang Zhongwei, Feng Yang, Zhao Zhongmeng, Zhao Han, Zhang Lu, Duan Yuanliang, Huang Zhipeng, Zhou Jian, Mou Chengyan
Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China.
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Animals (Basel). 2025 Jul 16;15(14):2107. doi: 10.3390/ani15142107.
Cold shock represents a prevalent but harmful environmental stress factor that poses significant threats to fish survival and reproductive success. In fish, the brain acts as a central regulator of thermoregulatory processes. Nevertheless, how different brain regions respond molecularly to cold exposure remains largely unknown. To address this, this study systematically investigated the effects of acute cold stress on five specific brain regions of using RNA-seq. The findings demonstrated that all five brain regions were significantly impacted by cold treatment, with the mesencephalon (MB) showing the most substantial changes. GO and KEGG enrichment analyses indicated that cold stress disrupted processes including gene expression regulation, circadian rhythms, and immune function within brain tissues. Through Weighted Gene Co-Expression Network Analysis (WGCNA), the MB was identified as the core responsive region, and the brain's reaction to cold stress was strongly correlated with circadian rhythm, spliceosome, and ubiquitination. In summary, our investigation demonstrates that the MB represents a principal region for cold stress response in , involving alterations in circadian clocks, immune function, and inflammatory responses, alongside suppression of gene expression processes and ubiquitination-mediated proteolysis.
冷休克是一种普遍存在但有害的环境应激因素，对鱼类生存和繁殖成功构成重大威胁。在鱼类中，大脑是体温调节过程的中央调节器。然而，不同脑区如何在分子水平上对冷暴露做出反应在很大程度上仍不清楚。为了解决这个问题，本研究使用RNA测序系统地研究了急性冷应激对[鱼类名称]五个特定脑区的影响。研究结果表明，所有五个脑区均受到冷处理的显著影响，其中中脑（MB）变化最为显著。基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，冷应激扰乱了脑组织内包括基因表达调控、昼夜节律和免疫功能在内的过程。通过加权基因共表达网络分析（WGCNA），确定中脑为核心反应区域，大脑对冷应激的反应与昼夜节律、剪接体和泛素化密切相关。总之，我们的研究表明，中脑是[鱼类名称]冷应激反应的主要区域，涉及昼夜节律、免疫功能和炎症反应的改变，以及基因表达过程的抑制和泛素化介导的蛋白水解。
