Tończyk Aleksandra, Niedziałkowska Katarzyna, Nowak-Lange Marta, Bernat Przemysław, Lisowska Katarzyna
Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha Street, 90-237 Lodz, Poland.
The BioMedChem Doctoral School, University of Lodz, Lodz Institutes of Polish Academy of Sciences, 21/23 Matejki Street, 90-237 Lodz, Poland.
Int J Mol Sci. 2025 Jul 10;26(14):6639. doi: 10.3390/ijms26146639.
The antimicrobial activity of silver nanoparticles (AgNPs) makes them a valuable tool in various industries. Recently, biosynthesis has become the preferred method for nanoparticle synthesis, and among organisms that can be used as AgNP producers, filamentous fungi have attracted the greatest interest. In particular, wood decay fungi are considered promising candidates for AgNP biosynthesis. Biogenic AgNPs have been proven to have strong antibacterial potential and antifungal activity. The aim of this study was to evaluate the antifungal potential of AgNPs synthesized using the brown-rot decay fungus DSM 9592 against four pathogenic fungal strains: , , and . Moreover, changes in the tested strains' lipidome and cell membrane properties induced by the presence of AgNPs were investigated. The results revealed that the obtained AgNPs exerted fungistatic activity against all the strains tested. , with a MIC value of 0.39 μg/mL obtained for all AgNP types, was found to be the most susceptible to the action of AgNPs. The lipidomic analysis revealed that the presence of AgNPs caused an increase in cell membrane fluidity in both and , and the mechanisms of response to AgNPs differed between the tested strains.
银纳米颗粒（AgNPs）的抗菌活性使其成为各行业中一种有价值的工具。最近，生物合成已成为纳米颗粒合成的首选方法，在可用作AgNP生产者的生物中，丝状真菌引起了最大关注。特别是，木材腐朽真菌被认为是AgNP生物合成的有希望的候选者。生物合成的AgNPs已被证明具有强大的抗菌潜力和抗真菌活性。本研究的目的是评估使用褐腐腐朽真菌DSM 9592合成的AgNPs对四种致病真菌菌株：[此处原文缺失菌株名称]、[此处原文缺失菌株名称]、[此处原文缺失菌株名称]和[此处原文缺失菌株名称]的抗真菌潜力。此外，还研究了AgNPs的存在对受试菌株脂质组和细胞膜特性的影响。结果表明，所获得的AgNPs对所有受试菌株均具有抑菌活性。[此处原文缺失菌株名称]对AgNPs的作用最为敏感，所有类型的AgNPs对其MIC值均为0.39μg/mL。脂质组分析表明，AgNPs的存在导致[此处原文缺失菌株名称]和[此处原文缺失菌株名称]的细胞膜流动性增加，受试菌株对AgNPs的反应机制有所不同。
