Al-Harrasi Khalid, Al-Habsi Nasser, Al-Kindi Mohamed A, Shi Linghong, Suleria Hafiz A R, Ashokkumar Muthupandian, Rahman Mohammad Shafiur
Department of Food Science and Nutrition, College of Agricultural and Marine Sciences, P.O. Box 34-123, Al-Khod 123, Muscat, Oman.
Department of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, P.O. Box 35-123, Al-Khod 123, Muscat, Oman.
Int J Mol Sci. 2025 Jul 11;26(14):6644. doi: 10.3390/ijms26146644.
Date pits are considered waste, and micronized date pit powder could be developed for use in foods and bio-products. In this study, micronized date pit powders were extracted by alcoholic sedimentation after ultrasound treatment. The control was considered untreated, i.e., without sonication. Six micronized fractions (i.e., three from control and three from treated) were prepared by three stages of alcoholic sedimentation. In the case of untreated date pit powder, the average particle size of the fractionated date pit powder (i.e., residue) from three stages of alcoholic sedimentation varied from 89 to 164 µm, while ultrasonic treatment showed variation from 39 to 65 µm. The average particle size of the supernatant fractions of untreated date pit powder varied from 22 to 63 µm, while ultrasonic treatment showed variation from 18 to 44 µm. Ultrasound treatment produced smaller particles. In all cases, Scanning Electron Microscopy (SEM) showed that supernatant fractions contained lumped particles compared to the residue fractions. Transmission Electron Microscopy (TEM) showed the presence of nanoparticles in all extracted fractions. Two glass transitions were observed in all fractions except for the residue from the first sedimentation stage. In addition, higher levels of degradation in the fractionated date pits could be achieved by ultrasonic treatment, as is evident from the Fourier Transform Infrared (FTIR) analysis.
枣核被视为废弃物，而微粉化枣核粉可开发用于食品和生物制品。在本研究中，微粉化枣核粉经超声处理后采用醇沉法提取。对照组为未处理组，即未进行超声处理。通过三个阶段的醇沉制备了六个微粉化级分（即三个来自对照组，三个来自处理组）。对于未处理的枣核粉，三个阶段醇沉得到的分级枣核粉（即残渣）的平均粒径在89至164微米之间，而超声处理后的平均粒径在39至65微米之间。未处理枣核粉上清液级分的平均粒径在22至63微米之间，而超声处理后的平均粒径在18至44微米之间。超声处理产生的颗粒更小。在所有情况下，扫描电子显微镜（SEM）显示，与残渣级分相比，上清液级分含有聚集的颗粒。透射电子显微镜（TEM）显示所有提取级分中均存在纳米颗粒。除了第一沉降阶段的残渣外，在所有级分中均观察到两个玻璃化转变。此外，傅里叶变换红外光谱（FTIR）分析表明，超声处理可使分级枣核中的降解水平更高。
