Tan Cuirong, Ban Dingyue, Li Haiyang, Wang Jinxing, Liu Baohui, Zhang Chunyu
Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Applied Botany & State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Int J Mol Sci. 2025 Jul 11;26(14):6689. doi: 10.3390/ijms26146689.
Deubiquitination plays a pivotal role in regulating plant responses to abiotic stress, growth, and development. Among the deubiquitinase (DUB) families, ubiquitin-specific proteases (UBPs) constitute the largest group. Despite this, limited research has been conducted on the functional characteristics of the gene family in soybean (). In this study, we identified 52 gene family members in soybean, all of which harbored UCH (ubiquitin C-terminal hydrolase) domains with short yet evolutionarily conserved Cys-box and His-box. These genes were phylogenetically classified into 14 distinct groups; genes within the same group shared analogous patterns of conserved domains and motifs. Moreover, a synteny analysis reveals that the family has undergone extensive gene duplication events and shares a close evolutionary relationship with . We conducted a focused analysis on , which is a gene exhibiting high expression levels in soybean seeds. Intriguingly, this gene exhibited several haplotypes in natural soybean varieties, with significant differences being observed in relation to seed traits, such as 100-seed weight, total fatty acid content, and protein content among different haplotypes. Collectively, the findings from this study provide a foundation for the functional characterization of genes, offering new insights into the regulatory network underlying seed development in soybean.
去泛素化在调节植物对非生物胁迫、生长和发育的反应中起着关键作用。在去泛素化酶（DUB）家族中，泛素特异性蛋白酶（UBP）构成了最大的群体。尽管如此，关于大豆中该基因家族功能特性的研究仍然有限。在本研究中，我们在大豆中鉴定出52个该基因家族成员，它们均含有UCH（泛素C末端水解酶）结构域以及短而进化保守的半胱氨酸框和组氨酸框。这些基因在系统发育上被分为14个不同的组；同一组内的基因具有相似的保守结构域和基序模式。此外，共线性分析表明该家族经历了广泛的基因复制事件，并且与……具有密切的进化关系。我们对……进行了重点分析，该基因在大豆种子中表现出高表达水平。有趣的是，该基因在天然大豆品种中表现出几种单倍型，不同单倍型在种子性状方面存在显著差异，如百粒重、总脂肪酸含量和蛋白质含量。总体而言，本研究结果为该基因的功能表征提供了基础，为大豆种子发育的调控网络提供了新的见解。
