Krongbaramee Tadkamol, Upara Chawin, Remy Matthew T, Jiang Long, Hu Jue, Tikkhanarak Kittiphoj, Cavalcanti Bruno, Sun Hongli, Teixeira Fabricio B, Hong Liu
Iowa Institute for Oral Health Research, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA.
Division of Endodontics, Department of Restorative Dentistry & Periodontology, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand.
Int J Mol Sci. 2025 Jul 14;26(14):6734. doi: 10.3390/ijms26146734.
enhances osteogenesis, modulates inflammation, and participates in dentin development. This study was to investigate the beneficial potential of in vital pulp therapy (VPT) by mitigating pulpitis and promoting dentin regeneration. We explored the variations in inflamed pulp tissues from patients with symptomatic irreversible pulpitis and primary human dental pulp-derived cells (DPCs) challenged with lipopolysaccharide (-LPS). We further assessed the functions of overexpression of on odontogenic differentiation, pulpal inflammation, and dentin regeneration in vitro and in vivo. Our findings revealed a noteworthy downregulation of expression in inflamed pulp tissues and primary human DPCs. Through the overexpression of via transfecting plasmid DNA (pDNA), we observed a substantial downregulation of proinflammatory cytokines interleukin (IL)-6 and IL-8 in human DPCs. Furthermore, this overexpression significantly enhanced the transcript and protein levels of odontogenic differentiation markers, including Runt-related transcription factor (Runx)2, osteocalcin (OCN), dentin matrix protein (DMP)1, and dentin sialophosphoprotein (DSPP). In a rat model of pulpitis induced by -LPS, we demonstrated notable benefits by local application of pDNA encoding delivered by CaCO-based nanoparticles to reduce pulpal inflammation and promote dentin formation. These results underscore the significant impact of locally applied in modulating pulpal inflammation and facilitating dentin repair, showcasing its ability to improve VPT outcomes.
增强骨生成、调节炎症并参与牙本质发育。本研究旨在通过减轻牙髓炎和促进牙本质再生来探讨[具体物质]在活髓治疗（VPT）中的有益潜力。我们研究了有症状的不可逆性牙髓炎患者发炎牙髓组织以及经脂多糖（-LPS）刺激的原代人牙髓来源细胞（DPCs）中[具体物质]的变化。我们进一步评估了[具体物质]过表达在体外和体内对牙源性分化、牙髓炎症和牙本质再生的作用。我们的研究结果显示，发炎牙髓组织和原代人DPCs中[具体物质]的表达显著下调。通过转染质粒DNA（pDNA）使[具体物质]过表达，我们观察到人类DPCs中促炎细胞因子白细胞介素（IL）-6和IL-8显著下调。此外，这种过表达显著提高了牙源性分化标志物的转录和蛋白水平，包括 runt 相关转录因子（Runx）2、骨钙素（OCN）、牙本质基质蛋白（DMP）1 和牙本质涎磷蛋白（DSPP）。在由-LPS诱导的牙髓炎大鼠模型中，我们证明了通过局部应用基于碳酸钙纳米颗粒递送的编码[具体物质]的pDNA可显著减轻牙髓炎症并促进牙本质形成。这些结果强调了局部应用[具体物质]在调节牙髓炎症和促进牙本质修复方面的显著影响，展示了其改善VPT效果的能力。
