Mitigates Pulpitis and Promotes Dentin Regeneration.

enhances osteogenesis, modulates inflammation, and participates in dentin development. This study was to investigate the beneficial potential of in vital pulp therapy (VPT) by mitigating pulpitis and promoting dentin regeneration. We explored the variations in inflamed pulp tissues from patients with symptomatic irreversible pulpitis and primary human dental pulp-derived cells (DPCs) challenged with lipopolysaccharide (-LPS). We further assessed the functions of overexpression of on odontogenic differentiation, pulpal inflammation, and dentin regeneration in vitro and in vivo. Our findings revealed a noteworthy downregulation of expression in inflamed pulp tissues and primary human DPCs. Through the overexpression of via transfecting plasmid DNA (pDNA), we observed a substantial downregulation of proinflammatory cytokines interleukin (IL)-6 and IL-8 in human DPCs. Furthermore, this overexpression significantly enhanced the transcript and protein levels of odontogenic differentiation markers, including Runt-related transcription factor (Runx)2, osteocalcin (OCN), dentin matrix protein (DMP)1, and dentin sialophosphoprotein (DSPP). In a rat model of pulpitis induced by -LPS, we demonstrated notable benefits by local application of pDNA encoding delivered by CaCO-based nanoparticles to reduce pulpal inflammation and promote dentin formation. These results underscore the significant impact of locally applied in modulating pulpal inflammation and facilitating dentin repair, showcasing its ability to improve VPT outcomes.