Czerewaty Michał, Ustianowski Łukasz, Kiełbowski Kajetan, Bakinowska Estera, Safranow Krzysztof, Tarnowski Maciej, Sroczyński Tomasz, Pawlik Andrzej
Department of Physiology, Pomeranian Medical University, 70-111 Szczecin, Poland.
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, 70-111 Szczecin, Poland.
Genes (Basel). 2025 Jul 20;16(7):844. doi: 10.3390/genes16070844.
BACKGROUND/OBJECTIVES: Gestational diabetes mellitus (GDM) is a common metabolic disorder in pregnant women. It can lead to several complications, such as preterm delivery, macrosomia, or metabolic disorders in newborns. Studies have revealed morphological and transcriptional differences between the placentas of patients with GDM and women with normal glucose tolerance. Sirtuins (SIRTs) are nicotinamide adenine dinucleotide-dependent deacetylases that interact with and regulate the activity of numerous proteins. However, little is known about their role in the pathogenesis of GDM. This study was performed to analyze the placental expression of SIRTs and investigate their correlations with clinical parameters.
GDM was diagnosed based on the 75 g oral glucose tolerance test in accordance with the criteria developed by the International Association of Diabetes and Pregnancy Study Groups. Placental tissues were collected, and the expression of and a reference gene (β-2 microglobulin) was analyzed.
The placental expression of and was elevated in women with GDM. However, there was no significant difference in expression between women with GDM and those with normal glucose tolerance. Furthermore, we found no significant correlations between , , and expression and clinical parameters.
The findings of this study demonstrate elevated expression of and in the placentas of women with GDM. Further studies are required to confirm our observations and demonstrate the precise role of these enzymes in GDM.
背景/目的：妊娠期糖尿病（GDM）是孕妇常见的代谢紊乱疾病。它可导致多种并发症，如早产、巨大儿或新生儿代谢紊乱。研究显示，GDM患者胎盘与糖耐量正常女性胎盘在形态和转录方面存在差异。沉默调节蛋白（SIRTs）是烟酰胺腺嘌呤二核苷酸依赖性脱乙酰酶，可与多种蛋白质相互作用并调节其活性。然而，它们在GDM发病机制中的作用尚不清楚。本研究旨在分析胎盘组织中SIRTs的表达情况，并探讨其与临床参数的相关性。
根据国际糖尿病与妊娠研究组制定的标准，采用75g口服葡萄糖耐量试验诊断GDM。收集胎盘组织，分析[具体蛋白名称未给出]和一个参考基因（β-2微球蛋白）的表达。
GDM女性胎盘组织中[具体蛋白名称未给出]和[另一具体蛋白名称未给出]的表达升高。然而，GDM女性与糖耐量正常女性的[具体蛋白名称未给出]表达无显著差异。此外，我们发现[具体蛋白名称未给出]、[另一具体蛋白名称未给出]的表达与临床参数之间无显著相关性。
本研究结果表明，GDM女性胎盘组织中[具体蛋白名称未给出]和[另一具体蛋白名称未给出]的表达升高。需要进一步研究以证实我们的观察结果，并阐明这些酶在GDM中的精确作用。
