Nabavi Akram Sadat, Ghoreishi Sayed Mehdi, Tajabadi Fateme
Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, I. R. Iran.
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, I. R. Iran.
Anal Methods. 2025 Aug 7;17(31):6397-6404. doi: 10.1039/d5ay00752f.
Orange peel is a rich source of nutrients and phytochemicals, widely used in medicines and foods. It contains flavonoids and antioxidants beneficial for health, including hesperidin (HES), a flavonoid abundant in citrus peels. This study used solid-phase extraction (SPE) and molecular spectrophotometry to extract and identify HES. The process is accurate, cost-effective, rapid, and environmentally friendly. HES was extracted using a hydroalcoholic method with MIL-101 (Cr), a metal-organic framework (MOF), as the adsorbent. MIL-101 (Cr) was prepared hydrothermally and characterized by Fourier transform infrared spectroscopy (FT-IR), field-emission scanning electron microscopy (FE-SEM), and X-ray diffraction (XRD). Experimental parameters such as sample concentration, adsorbent dosage, pH, and solvent type were optimized. HES was desorbed using acetonitrile, and absorption was measured at 285 nm. The limit of detection (LOD) and limit of quantification (LOQ) for HES were 0.145 and 0.530 μg mL, respectively. The method exhibited good reproducibility with less than 2% RSD. This study successfully demonstrated the use of MIL-101 (Cr) in HES extraction, offering a rapid, specific, accurate, and cost-effective analysis method.
橙皮是丰富的营养物质和植物化学物质来源，广泛应用于医药和食品领域。它含有对健康有益的类黄酮和抗氧化剂，包括橙皮苷（HES），一种在柑橘皮中含量丰富的类黄酮。本研究采用固相萃取（SPE）和分子分光光度法提取并鉴定HES。该方法准确、经济高效、快速且环保。使用水醇法，以金属有机框架（MOF）MIL-101（Cr）作为吸附剂提取HES。通过水热法制备MIL-101（Cr），并采用傅里叶变换红外光谱（FT-IR）、场发射扫描电子显微镜（FE-SEM）和X射线衍射（XRD）对其进行表征。对样品浓度、吸附剂用量、pH值和溶剂类型等实验参数进行了优化。用乙腈解吸HES，并在285nm处测量吸光度。HES的检测限（LOD）和定量限（LOQ）分别为0.145和0.530μg/mL。该方法具有良好的重现性，相对标准偏差（RSD）小于2%。本研究成功证明了MIL-101（Cr）在HES提取中的应用，提供了一种快速、特异、准确且经济高效的分析方法。
