Cambridge G W, Parsons J F, Friend J V, Jones P A
Agents Actions. 1985 Sep;16(6):548-51. doi: 10.1007/BF01983661.
Macrophages were exposed to lignocaine in perifused and non-perifused cultures using media with or without foetal calf serum. Effects on morphology, viability, phagocytosis and the release of enzymes were assessed. During the period of contact with lignocaine there was a selective release of beta-glucuronidase. After washing, enzyme release continued over a period of 7 hours and, in the absence of foetal calf serum, a decrease in the total beta-glucuronidase content was found in non-perifused cultures. Although lignocaine-treated cells phagocytosed particulates the rate of enzyme release was reduced compared with normal cells when subsequently exposed to quartz.
在有或没有胎牛血清的培养基中，使巨噬细胞在灌流培养和非灌流培养中接触利多卡因。评估其对形态、活力、吞噬作用和酶释放的影响。在与利多卡因接触期间，有β-葡萄糖醛酸酶的选择性释放。洗涤后，酶释放持续7小时，并且在没有胎牛血清的情况下，在非灌流培养物中发现总β-葡萄糖醛酸酶含量降低。尽管用利多卡因处理的细胞吞噬颗粒，但与正常细胞相比，随后暴露于石英时酶释放速率降低。
