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体外细胞灌注。I. 用于连续观察细胞和分析灌注液的系统，适用于分离肥大细胞和巨噬细胞的研究。

In vitro cell perfusion. I. System for continuous observation of cells and analysis of perfusion fluid suitable for isolated mast cell and macrophage studies.

作者信息

Parsons J F, Safford R J, Friend J V, Philp J

出版信息

J Pharmacol Methods. 1982 Aug;8(1):73-89. doi: 10.1016/0160-5402(82)90009-2.

DOI:10.1016/0160-5402(82)90009-2
PMID:6181346
Abstract

A system is described in which cells are perfused continuously with appropriate medium while simultaneously recording any change in cell morphology together with assay of released products (enzymes and mediators) following immunologic or non-immunologic perturbation. The released products in the perfusate are monitored continuously by automated fluorimetric assay procedures. The system is particularly applicable to investigate or toxicological studies of isolated mast cells or cultured macrophages.

摘要

本文描述了一种系统，在该系统中，细胞在持续灌注合适培养基的同时，记录细胞形态的任何变化，并对免疫或非免疫扰动后释放的产物（酶和介质）进行测定。灌注液中的释放产物通过自动荧光分析程序进行连续监测。该系统特别适用于对分离的肥大细胞或培养的巨噬细胞进行研究或毒理学研究。

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In vitro cell perfusion. I. System for continuous observation of cells and analysis of perfusion fluid suitable for isolated mast cell and macrophage studies.体外细胞灌注。I. 用于连续观察细胞和分析灌注液的系统，适用于分离肥大细胞和巨噬细胞的研究。
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