Protopopov Vladimir A, Sekunov Alexey V, Toropova Tatiana V, Bryndina Irina G, Burganova Dilyara N, Milaev Vyacheslav B, Sharafislamova Mariya B, Pozdeev Sergey V, Moskalev Alexey A
Department of Pathophysiology and Immunology, Izhevsk State Medical Academy, Kommunarov Street, Izhevsk, 426000, Russia.
Depatment of Research and Development, Limited Liability Company "Spring", Vasilkovaya Street, Izhevsk, 426000, Russia.
Biogerontology. 2025 Jul 29;26(4):150. doi: 10.1007/s10522-025-10293-0.
This pilot study aimed to evaluate the impact of a diet supplemented with potential geroprotectors on metabolic and aging-associated markers in domestic cats. A total of 53 adult domestic cats were randomly assigned into two groups: a control diet (CD) and a geroprotective diet (GD) containing five plant-based extracts (silymarin, green tea, grape seed, curcumin, and grapefruit seed). Animals were fed the respective diets for three months. Body weight and body condition score were recorded before and after the feeding period. Blood samples were analyzed for standard biochemical parameters. Peripheral blood mononuclear cells were isolated to assess the expression of markers associated with inflammation (NF-κB, IL-1β), senescence (p16), and metabolic regulation (SIRT1, Klotho, RAGE) by Western blot. Both diets supported adequate body weight and condition. The GD group demonstrated a significant reduction in serum creatinine and a marked decrease in inflammatory and senescence markers (lower RAGE, p16, phospho-NF-κB) compared to baseline. SIRT1 levels were significantly upregulated only in the GD group, suggesting a possible protective mechanism against age-related changes. These data reveal a mechanistic link between dietary geroprotector supplementation and the modulation of key molecular hallmarks of aging in felines, highlighting conserved pathways of aging across mammalian species and establishing a foundation for translational interventions targeting age-associated decline in companion animals. Further long-term and larger-scale studies are required to confirm and extend these observations.
这项初步研究旨在评估添加潜在老年保护剂的饮食对家猫代谢和衰老相关标志物的影响。总共53只成年家猫被随机分为两组：对照组饮食（CD）和含有五种植物提取物（水飞蓟素、绿茶、葡萄籽、姜黄素和葡萄柚籽）的老年保护饮食（GD）。动物分别喂食各自的饮食三个月。在喂食期前后记录体重和身体状况评分。分析血样的标准生化参数。分离外周血单核细胞，通过蛋白质印迹法评估与炎症（NF-κB、IL-1β）、衰老（p16）和代谢调节（SIRT1、Klotho、RAGE）相关的标志物的表达。两种饮食都能维持适当的体重和身体状况。与基线相比，GD组血清肌酐显著降低，炎症和衰老标志物（RAGE、p16、磷酸化NF-κB降低）明显减少。SIRT1水平仅在GD组中显著上调，表明可能存在针对与年龄相关变化的保护机制。这些数据揭示了饮食中添加老年保护剂与猫科动物衰老关键分子特征调节之间的机制联系，突出了跨哺乳动物物种衰老的保守途径，并为针对伴侣动物年龄相关衰退的转化干预奠定了基础。需要进一步的长期和大规模研究来证实和扩展这些观察结果。
