Stanislavsky E S, Bogdanova V V, Kirpatovsky I D, Zhvanetskaya M I
J Hyg Epidemiol Microbiol Immunol. 1977;21(1):84-94.
A substance, inhibiting the production of haemolysins against sheep erythrocytes in mice was isolated from two non-pathogenic strains of E. coli, 020:K4 and M-17, by the methods of differential centrifugation and gel filtration. Spectrophotometric studies and chemical analysis have shown the isolated substance to be glycolipid. The immunodepressive substance is localized in the cytoplasm of the microbial cell. The isolated and partly purified immunodepressive substance did not contain any admixture of O-antigen (endotoxin) of the cell wall or of antigens giving cross reactions with sheep erythrocytes. The isolated substance exhibited weak antigenic properties and was not toxic for mice when administered in a dose of 2 mg (dry weight).
通过差速离心和凝胶过滤法，从两株非致病性大肠杆菌O20:K4和M-17中分离出一种能抑制小鼠体内针对绵羊红细胞溶血素产生的物质。分光光度研究和化学分析表明，分离出的物质是糖脂。免疫抑制物质定位于微生物细胞的细胞质中。分离并部分纯化的免疫抑制物质不含细胞壁O抗原（内毒素）或与绵羊红细胞产生交叉反应的抗原的任何混合物。分离出的物质表现出较弱的抗原特性，以2毫克（干重）的剂量给小鼠注射时无毒。
