Mahmoud Hadeer Saied, Eldesoky Noha Abdel-Rahman, Shaker Olfat G, El-Husseiny Ahmed A
Department of Biochemistry, Faculty of Pharmacy, Egyptian Russian University, Badr City, Cairo, 11829, Egypt.
Biochemistry and Molecular Biology Department, Faculty of Pharmacy (Boys and Girls), Al-Azhar University, Nasr City, Cairo, Egypt.
Sci Rep. 2025 Jul 29;15(1):27638. doi: 10.1038/s41598-025-12963-y.
Early pancreatic cancer (PC) identification and management has gained much clinical and research attention. Recent reports have demonstrated the important function of circulating noncoding RNAs (ncRNAs) in the diagnosis and prognosis of malignancies. However, the clinical value of serum ncRNAs in PC has not been fully clarified. Hence, we investigated serum levels of long-ncRNA NEAT1 and miR-129-5p in PC cases, exploring their relationship with related targets BCL2 and TGF-β1. Serum NEAT1 and miR-129-5p levels were evaluated in 60 treatment-naïve PC cases and 30 apparent healthy individuals by RT-PCR. Besides, serum TGF-β1 and BCL2 levels were measured by ELISA, whereas CA19-9 and CEA were measured utilizing the chemiluminescence technique. We demonstrated that serum NEAT1, BCL2, and TGF-β1 levels were significantly upregulated, whereas serum levels of miR-129-5p were markedly reduced in PC cases compared to controls. miR-129-5p had a higher diagnostic value for PC than NEAT1, with specificity, sensitivity, and AUC of 100%, 95%, and 0.96 versus 93.3%, 83.3%, and 0.89, respectively. Moreover, miR-129-5p had a higher AUC than CA19-9 and CEA. Additionally, serum miR-129-5p was significantly downregulated in PC cases with T3 or T4 stages compared to those with T2 stage & negatively correlated with NEAT1, BCL2, and TGF-β1, whereas NEAT1 was notably positively correlated with BCL2 and TGF-β1. Collectively, serum NEAT1 and miR-129-5p could be beneficial biomarkers for the detection of PC. Also, our findings accentuate the correlation between NEAT1, miR-129-5p, BCL2, and TGF-β1 and provide PC therapeutic targets.
早期胰腺癌（PC）的识别与管理已受到众多临床和研究关注。近期报告显示循环非编码RNA（ncRNAs）在恶性肿瘤的诊断和预后中具有重要作用。然而，血清ncRNAs在PC中的临床价值尚未完全阐明。因此，我们研究了PC患者血清中长链非编码RNA NEAT1和miR-129-5p的水平，探讨它们与相关靶点BCL2和TGF-β1的关系。通过逆转录聚合酶链反应（RT-PCR）评估了60例未经治疗的PC患者和30名明显健康个体的血清NEAT1和miR-129-5p水平。此外，采用酶联免疫吸附测定（ELISA）法检测血清TGF-β1和BCL2水平，而采用化学发光技术检测CA19-9和癌胚抗原（CEA）。我们发现，与对照组相比，PC患者血清中NEAT1、BCL2和TGF-β1水平显著上调，而miR-129-5p血清水平明显降低。miR-129-5p对PC的诊断价值高于NEAT1，其特异性、敏感性和曲线下面积（AUC）分别为100%、95%和0.96，而NEAT1分别为93.3%、83.3%和0.89。此外，miR-129-5p的AUC高于CA19-9和CEA。另外，与T2期PC患者相比，T3或T4期PC患者血清miR-129-5p明显下调，且与NEAT1、BCL2和TGF-β1呈负相关，而NEAT1与BCL2和TGF-β1显著正相关。总体而言，血清NEAT1和miR-129-5p可能是检测PC的有益生物标志物。此外，我们的研究结果强调了NEAT1、miR-129-5p、BCL2和TGF-β1之间的相关性，并提供了PC治疗靶点。
