Perera Thejanee, De Silva Ishani, Bandara Pavithri, Bowatte Asanka, Rathnayake Dinithi, Perera Shanika, Sumathipala Saranga, Muthugala Rohitha
Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, 00300, Sri Lanka.
Teaching Hospital Peradeniya, Peradeniya, 20400, Sri Lanka.
BMC Genom Data. 2025 Jul 29;26(1):52. doi: 10.1186/s12863-025-01343-0.
Respiratory Syncytial Virus (RSV) is a major cause of acute respiratory tract infections (ARTI) worldwide, with seasonal outbreaks often influenced by climatic factors. This study investigates the genetic diversity and phylogenetic relationships of RSV strains circulated in Sri Lanka between 2022 December and 2024 February.
Subtyping of 12 RSV-positive samples identified both RSV A and B, with full-length F gene sequences obtained for four samples. Phylogenetic analysis revealed clustering with globally circulating strains. Mutation profiling highlighted key amino acid substitutions, including a rare mutation of N116D in the F protein, which may have functional implications. The limited sample size necessitates further studies with broader geographic coverage for more robust conclusions.
This study provides insights into the genetic diversity of RSV F gene in Sri Lanka.
呼吸道合胞病毒（RSV）是全球急性呼吸道感染（ARTI）的主要病因，季节性暴发常受气候因素影响。本研究调查了2022年12月至2024年2月在斯里兰卡传播的RSV毒株的遗传多样性和系统发育关系。
对12份RSV阳性样本进行亚型分析，鉴定出RSV A和B两种亚型，并获得了4个样本的全长F基因序列。系统发育分析显示与全球流行毒株聚类。突变谱分析突出了关键氨基酸替换，包括F蛋白中罕见的N116D突变，这可能具有功能意义。样本量有限，需要进一步开展地理覆盖范围更广的研究以得出更可靠的结论。
本研究为斯里兰卡RSV F基因的遗传多样性提供了见解。
