Mashzhan Akzhigit, Smekenov Izat, Bakiyev Serik, Utegenova Kalamkas, Samatkyzy Diana, Daniyarov Asset, Kairov Ulykbek, Sarbassov Dos, Bissenbaev Amangeldy
Scientific Research Institute of Biology and Biotechnology Problems, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.
Almaty Branch of the National Center for Biotechnology, Central Reference Laboratory, Zhahanger St. 14, Almaty 050054, Kazakhstan.
Microorganisms. 2025 Jul 17;13(7):1680. doi: 10.3390/microorganisms13071680.
spp. are opportunistic pathogens that are widely distributed in water sources, with several species being associated with fish and human diseases. We have previously identified an AB005 isolate from diseased . This isolate was identified as based on the 16S rRNA and gene sequences. However, this novel strain does not produce indole and tested negative for ornithine decarboxylase and d-xylose fermentation-differences that set it apart from typical strains. In the present study, this strain was subjected to whole-genome sequencing and compared with the genomes of the type strain ( ATCC 7966) and other spp. Comprehensive genome analysis suggests that AB005 represents a distinct species within the genus. The draft genome of the AB005 strain comprises 4,780,815 base pairs with a GC content of 61.2% and contains 6104 predicted protein-coding sequences along with numerous genes implicated in antibiotic resistance. The core/pan-genome analysis reveals extensive genetic diversity, indicative of a dynamic genomic structure. These findings collectively underscore the taxonomic distinction of the AB005 strain as a novel species and highlight its potential pathogenic implications in aquaculture and public health settings.
某些物种是机会致病菌，广泛分布于水源中，有几种物种与鱼类和人类疾病有关。我们之前从患病个体中鉴定出了AB005分离株。根据16S rRNA和（此处原文缺失相关基因名称）基因序列，该分离株被鉴定为（此处原文缺失相关物种名称）。然而，这种新菌株不产生吲哚，鸟氨酸脱羧酶和d -木糖发酵检测呈阴性，这些差异使其与典型的（此处原文缺失相关物种名称）菌株不同。在本研究中，对该菌株进行了全基因组测序，并与模式菌株（ATCC 7966）和其他（此处原文缺失相关物种名称）物种的基因组进行了比较。全面的基因组分析表明，AB005代表了该属内一个独特的物种。AB005菌株的基因组草图包含4,780,815个碱基对，GC含量为61.2%，包含6104个预测的蛋白质编码序列以及许多与抗生素抗性相关的基因。核心/泛基因组分析揭示了广泛的遗传多样性，表明基因组结构具有动态性。这些发现共同强调了AB005菌株作为一个新物种的分类学区别，并突出了其在水产养殖和公共卫生环境中的潜在致病意义。
