Zhang Minmin, Wang Xiao, Su Xiaoling, Yimamu Aidiya, Li Lanjuan, Sun Zeyu
Jinan Microecological Biomedicine Shandong Laboratory, Jinan 250117, China.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
Pathogens. 2025 Jun 30;14(7):645. doi: 10.3390/pathogens14070645.
Infectious diseases remain a leading cause of mortality worldwide. The pathogenesis that comprises infection, focal inflammation, and immuno-response, typically occurs in one or multiple organs or tissues. Analysis of the molecular composition of affected tissues with their spatial context is pivotal to elucidate the underlying disease mechanisms and to develop accurate diagnostic strategies. In recent years, mass spectrometry imaging (MSI) technology has achieved significant advancements and has emerged as an powerful tool for tissue-based molecular exploration with high molecular specificity and spatial resolution. Although MSI has been rapidly adopted in numerous branches of biomedical research, its application in the field of infectious diseases research is still in its early stages. With this in mind, this review aims to familiarize infectious disease researchers with the advantages and diverse applications of MSI. Additionally, we delineate several existing technical challenges, application pitfalls, and the potential solutions to overcome these challenges.
传染病仍然是全球主要的死亡原因。由感染、局灶性炎症和免疫反应组成的发病机制通常发生在一个或多个器官或组织中。分析受影响组织的分子组成及其空间背景对于阐明潜在的疾病机制和制定准确的诊断策略至关重要。近年来，质谱成像（MSI）技术取得了重大进展，并已成为一种具有高分子特异性和空间分辨率的强大的基于组织的分子探索工具。尽管MSI已在生物医学研究的众多分支中迅速得到应用，但其在传染病研究领域的应用仍处于早期阶段。有鉴于此，本综述旨在使传染病研究人员熟悉MSI的优势和多样应用。此外，我们还阐述了几个现有的技术挑战、应用陷阱以及克服这些挑战 的潜在解决方案。
