Smolec Dominika, Aptekorz Małgorzata, Filipczyk Łukasz, Gofron Zygmunt, Zostawa Jacek, Smolec Robert, Wąsik Tomasz J, Ekiel Alicja
Department of Medical Microbiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-752 Katowice, Poland.
Department of Histology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-752 Katowice, Poland.
Pathogens. 2025 Jul 8;14(7):670. doi: 10.3390/pathogens14070670.
Urease-positive urogenital mycoplasmas are considered to be responsible for the formation of urinary stones. They are usually a part of the normal flora in the human urogenital tract, causing asymptomatic infections. However, many symptomatic infections with these bacteria have been reported. is recognized as a cause of male urethritis and other common genitourinary diseases. The role of other urogenital mycoplasmas is still unclear. The aim of this study was to estimate the quantitative prevalence of spp., and in men with urolithiasis using quantitative real-time PCR (qPCR). The study group comprised 100 men with urolithiasis. A total of 60 men were included in the control group. Urogenital mycoplasma DNA in urine samples was detected significantly more often among men with urolithiasis than in healthy subjects-43.0% vs. 26.6%, = 0.0382, respectively. The majority of positive results (38/43) concerned species, the frequency of which was higher in the study group (38.0% (38/100)) than in the control group (23.3% (14/60)), = 0.0552. The median concentration of DNA was higher in the study group compared with the control, = 0.5714. However, further studies are needed to confirm the usefulness of quantitative studies in determining the role of urogenital mycoplasmas in pathology.
脲酶阳性泌尿生殖道支原体被认为是尿路结石形成的原因。它们通常是人类泌尿生殖道正常菌群的一部分，可引起无症状感染。然而，已有许多关于这些细菌引起症状性感染的报道。 被认为是男性尿道炎和其他常见泌尿生殖系统疾病的病因。其他泌尿生殖道支原体的作用仍不清楚。本研究的目的是使用定量实时PCR（qPCR）估计尿路结石患者中 spp.、 和 的定量流行情况。研究组包括100例尿路结石患者。对照组共纳入60名男性。尿路结石患者尿液样本中泌尿生殖道支原体DNA的检测频率显著高于健康受试者，分别为43.0%和26.6%， = 0.0382。大多数阳性结果（38/43）与 种有关，其在研究组中的频率（38.0%（38/100））高于对照组（23.3%（14/60））， = 0.0552。研究组中 DNA的中位数浓度高于对照组， = 0.5714。然而，需要进一步研究以证实定量研究在确定泌尿生殖道支原体在病理学中的作用方面的实用性。
