Palowski Amanda, Domingues Francisco, Lopez Othmar, Holcombe Nicole, Shurson Gerald, Schroeder Declan C
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
Anitox Corporation, Lawrenceville, GA 30043, USA.
Pathogens. 2025 Jul 8;14(7):672. doi: 10.3390/pathogens14070672.
The objective of this study was to evaluate the time-course of incubation for the potential preventative mitigation of megaviruses using Termin-8 (a formaldehyde-based product) and Finio (non-formaldehyde solution) from Anitox. Emiliania huxleyi virus (EhV), an algal surrogate for African swine fever virus (ASFV), was treated with the recommended concentrations of Termin-8 (0.1% to 0.3%) and Finio (0.05% to 0.2%), and both viability qPCR (V-qPCR) and standard PCR (S-qPCR) were used to quantify EhV concentrations at 1 h, 5 h, 24 h and day 7 post-inoculation. Overall, Finio, and to a lesser extent Termin-8, at their highest treatment concentrations, showed the greatest log reduction of 4.5 and 2 log units, respectively, at 1 h post-inoculation. Although Termin-8 efficacy did not improve with time, due to its fixing of viral particles and rendering them non-infectious, treatment with Finio showed 100% viable viral inactivation (>5 log reduction units) at the lowest concentration after 7 days of exposure. Our results demonstrate that both Termin-8 and Finio can be used as effective chemical mitigants against megaviruses such as EhV and ASFV and can be used as effective preventive or mitigation strategies to prevent the transmission of ASFV by reducing particle viability in contaminated feed, although additional research is warranted.
本研究的目的是评估使用来自Anitox的Termin-8（一种基于甲醛的产品）和Finio（非甲醛溶液）对巨型病毒进行潜在预防性缓解的孵育时间进程。以赫氏圆石藻病毒（EhV）作为非洲猪瘟病毒（ASFV）的藻类替代物，用推荐浓度的Termin-8（0.1%至0.3%）和Finio（0.05%至0.2%）进行处理，并在接种后1小时、5小时、24小时和第7天使用活力定量聚合酶链反应（V-qPCR）和标准聚合酶链反应（S-qPCR）来量化EhV浓度。总体而言，Finio在最高处理浓度下，在接种后1小时显示出最大的对数减少量，为4.5个对数单位，Termin-8在较小程度上显示为2个对数单位。尽管Termin-8的功效不会随时间提高，因为它会固定病毒颗粒并使其失去传染性，但在暴露7天后，Finio在最低浓度下显示出100%的病毒活性失活（>5个对数减少单位）。我们的结果表明，Termin-8和Finio都可以用作针对EhV和ASFV等巨型病毒的有效化学缓解剂，并且可以通过降低受污染饲料中颗粒的活力用作预防ASFV传播的有效预防或缓解策略，不过仍需要进行更多研究。
