Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Bijvoet Centre for Biomolecular Research, Utrecht University, Utrecht, Netherlands.
Sci Adv. 2024 Apr 12;10(15):eadk1954. doi: 10.1126/sciadv.adk1954. Epub 2024 Apr 10.
The globally distributed marine alga has cooling effect on the Earth's climate. The population density of is restricted by viruses, including virus 201 (EhV-201). Despite the impact of viruses on the climate, there is limited information about their structure and replication. Here, we show that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane. EhV-201 virions infect by using fivefold vertices to bind to and fuse the virus' inner membrane with the cell plasma membrane. Progeny virions assemble in the cytoplasm at the surface of endoplasmic reticulum-derived membrane segments. Genome packaging initiates synchronously with the capsid assembly and completes through an aperture in the forming capsid. The genome-filled capsids acquire an outer membrane by budding into intracellular vesicles. EhV-201 infection induces a loss of surface protective layers from cells, which enables the continuous release of virions by exocytosis.
全球分布的海洋藻类对地球气候具有冷却作用。 的种群密度受到病毒的限制，包括 201 病毒（EhV-201）。尽管 病毒对气候有影响，但关于它们的结构和复制的信息有限。在这里，我们表明 EhV-201 病毒粒子内部的 dsDNA 基因组被内膜、衣壳和外膜保护。EhV-201 病毒粒子通过五倍体顶点感染 ，使病毒的内膜与细胞质膜融合。子代病毒粒子在内质网衍生的膜段表面的细胞质中组装。基因组包装与衣壳组装同步开始，并通过形成衣壳中的一个孔完成。充满基因组的衣壳通过出芽进入细胞内小泡获得外膜。EhV-201 感染诱导 细胞表面保护层的丧失，这使得病毒粒子通过胞吐作用持续释放。
