Lee Seunghwa, Kim Aram, Koo Jimo, Kim Yunsik, Choi Sunglim, Cho Jin Cheol
R&D Center, CHA Meditech. Co., Ltd., 119 Techno 2-ro, Yuseong-gu, Daejeon 34116, Republic of Korea.
Department of Biomedical Science, School of Medicine, CHA University, Seongnam 13488, Republic of Korea.
Polymers (Basel). 2025 Jul 8;17(14):1892. doi: 10.3390/polym17141892.
In this study, we designed an injectable skin-rejuvenating formulation based on polyethylene glycol-polycaprolactone-polyethylene glycol (PEG-PCL-PEG) copolymers to provide a synergistic combination of biocompatibility, antioxidative capacity, and regenerative potential. Through the systematic optimization of the precursor molar ratio and molecular weight, well-defined PEG-PCL-PEG copolymers were synthesized and structurally characterized using gel permeation chromatography (GPC), proton nuclear magnetic resonance (H-NMR), and Fourier transform infrared (FT-IR) spectroscopy. An optimized precipitation and drying protocol effectively reduced residual solvents, as confirmed by gas chromatography (GC). Idebenone was incorporated as an antioxidant to prevent skin aging, while hyaluronic acid (HA), L-arginine, and glycerin were included to promote collagen regeneration. In vitro assays demonstrated that idebenone-loaded samples exhibited prolonged intracellular antioxidant activity with low cytotoxicity. The collagen-promoting formulation, containing HA, glycerin, and L-arginine, enhanced the expression of transforming growth factor-β (TGF-β) and type III collagen (COL3) while suppressing inflammatory genes, suggesting a favorable environment for extracellular matrix remodeling. In vivo evaluation corroborated these outcomes, showing angiogenesis, collagen reorganization, and progressive dermal thickness. Histological analysis further confirmed sustained matrix regeneration and tissue integration. These results highlight the potential of PEG-PCL-PEG-based injectables as a multifunctional platform for collagen regeneration, offering a promising strategy for both cosmetic and clinical applications.
在本研究中，我们基于聚乙二醇-聚己内酯-聚乙二醇（PEG-PCL-PEG）共聚物设计了一种可注射的皮肤修复配方，以提供生物相容性、抗氧化能力和再生潜力的协同组合。通过对前体摩尔比和分子量的系统优化，合成了明确的PEG-PCL-PEG共聚物，并使用凝胶渗透色谱法（GPC）、质子核磁共振（H-NMR）和傅里叶变换红外（FT-IR）光谱对其结构进行了表征。气相色谱法（GC）证实，优化的沉淀和干燥方案有效减少了残留溶剂。艾地苯醌作为抗氧化剂被加入以防止皮肤衰老，同时加入透明质酸（HA）、L-精氨酸和甘油以促进胶原蛋白再生。体外试验表明，负载艾地苯醌的样品表现出延长的细胞内抗氧化活性且细胞毒性低。含有HA、甘油和L-精氨酸的促进胶原蛋白生成的配方增强了转化生长因子-β（TGF-β）和III型胶原蛋白（COL3）的表达，同时抑制了炎症基因，表明为细胞外基质重塑提供了有利环境。体内评估证实了这些结果，显示出血管生成、胶原蛋白重组和真皮厚度逐渐增加。组织学分析进一步证实了持续的基质再生和组织整合。这些结果突出了基于PEG-PCL-PEG的可注射剂作为胶原蛋白再生多功能平台的潜力，为美容和临床应用提供了一种有前景的策略。
