Jing Yan, Wang Jinyu, He Yating, Liu Zedan, Liu Xiaolan
College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, China.
Key Laboratory of Corn Deep Processing Theory and Technology of Heilongjiang Province, Qiqihar 161006, China.
Nutrients. 2025 Jul 18;17(14):2358. doi: 10.3390/nu17142358.
This study investigated the antithrombotic properties of a fibrinolytic enzyme (CFE) purified from the culture supernatant of using a zebrafish thrombosis model.
A phenylhydrazine-induced thrombosis model was employed to evaluate the in vivo thrombolytic efficacy and mechanisms of CFE.
CFE significantly attenuated thrombogenesis by inhibiting erythrocyte aggregation in the caudal vessels, reducing staining intensity (3.61-fold decrease) and staining area (3.89-fold decrease). Concurrently, CFE enhanced cardiac hemodynamics, increasing erythrocyte staining intensity (9.29-fold) and staining area (5.55-fold) while achieving an 85.19% thrombosis inhibition rate. Behavioral analysis confirmed improved motility, with CFE-treated zebrafish exhibiting 2.23-fold increases in total movement distance and average speed, alongside a 3.59-fold extension in active movement duration. Mechanistically, ELISA revealed the multi-pathway activity of CFE, promoting fibrinolysis through reductions in plasminogen, fibrinogen, and D-dimer; inhibiting platelet activation via downregulation of prostaglandin-endoperoxide synthase (PTGS), thromboxane A2 (TXA2), P-selectin, and von Willebrand factor (vWF); and modulating coagulation cascades through elevated protein C and tissue factor pathway inhibitor (TFPI) with concurrent suppression of coagulation factor VII (FVII).
These results indicate that the fibrinolytic enzyme CFE, derived from , exerts potent antithrombotic effects, supporting its potential as a basis for fungal-derived natural antithrombotic functional food ingredients.
本研究使用斑马鱼血栓形成模型，研究了从[具体来源]培养上清液中纯化的纤溶酶（CFE）的抗血栓特性。
采用苯肼诱导的血栓形成模型评估CFE的体内溶栓效果及机制。
CFE通过抑制尾血管中的红细胞聚集、降低染色强度（降低3.61倍）和染色面积（降低3.89倍），显著减轻血栓形成。同时，CFE增强了心脏血流动力学，增加了红细胞染色强度（增加9.29倍）和染色面积（增加5.55倍），同时实现了85.19%的血栓抑制率。行为分析证实运动能力得到改善，经CFE处理的斑马鱼总移动距离和平均速度增加了2.23倍，主动运动持续时间延长了3.59倍。从机制上讲，ELISA显示CFE具有多途径活性，通过降低纤溶酶原、纤维蛋白原和D-二聚体促进纤维蛋白溶解；通过下调前列腺素内过氧化物合酶（PTGS）、血栓素A2（TXA2）、P-选择素和血管性血友病因子（vWF）抑制血小板活化；通过提高蛋白C和组织因子途径抑制剂（TFPI）并同时抑制凝血因子VII（FVII）来调节凝血级联反应。
这些结果表明，源自[具体来源]的纤溶酶CFE具有强大的抗血栓作用，支持其作为真菌来源的天然抗血栓功能性食品成分基础的潜力。
