Pechkova Eugenia, Fiordoro Stefano, Izzotti Alberto, Riekel Christian
Laboratories of Biophysics and Nanotechnology, Department of Experimental Medicine (DIMES), University of Genova Medical School, Via A. Pastore, 3, 16132 Genova, Italy.
Department of Health Science (DISSAL), University of Genova Medical School, 16132 Genova, Italy.
Molecules. 2025 Jul 9;30(14):2908. doi: 10.3390/molecules30142908.
Amyloids are protein-based biomaterials composed of fibrils with cross-β cores. Previously only associated with degenerative diseases, such as Parkinson's disease, Alzheimer's disease, and diabetes, amyloids remain active and functional both in vivo and in vitro conditions, enabling a variety of applications in medicine, nanotechnology, and biotechnology. This review aims to review the most advanced methods for amyloid fibril structural studies, with special attention on amyloid thin films. Selected advances of biomedical and biotechnological relevance will be outlined, and perspectives for future studies in the context of ongoing methodological progress will be discussed.
淀粉样蛋白是由具有交叉β核心的原纤维组成的基于蛋白质的生物材料。以前仅与退行性疾病相关，如帕金森病、阿尔茨海默病和糖尿病，淀粉样蛋白在体内和体外条件下均保持活性和功能性，从而在医学、纳米技术和生物技术领域有多种应用。本综述旨在回顾淀粉样原纤维结构研究的最先进方法，特别关注淀粉样蛋白薄膜。将概述具有生物医学和生物技术相关性的选定进展，并讨论在当前方法学进展背景下未来研究的前景。
