Zheng Feixiong, Xu Zhangting, Deng Xiaoji, Wang Xiaoyuan, Sun Yiming, Shen Xiaoxia, Yu Zhenming
School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Songyang Institute, Zhejiang Chinese Medical University, Lishui 323400, China.
Plants (Basel). 2025 Jul 8;14(14):2094. doi: 10.3390/plants14142094.
Flavonoids are key bioactive compounds in plants that play important defense roles against abiotic stress and are involved in plant growth and development. Methyl jasmonate (MeJA) is a significant growth regulator that promotes the accumulation of flavonoids in a variety of plants, but the effect of MeJA in remains poorly understood. In the present study, the flavonoid content was significantly increased after MeJA treatment and peaked at 6 h post-treatment. A total of 31,931 genes were identified using transcriptome, and 6484 DEGs were identified at 6 h post-treatment. Through GO and KEGG enrichment analysis, it was shown that DEGs were primarily enriched in phenylpropanoid biosynthesis pathways. Based on the putative transcription factors derived from DEGs, growth-regulating factor (GRF), a transcription factor potentially linking MeJA signaling to flavonoid accumulation and participating in plant growth and stress responses, was further identified. A total of 20 Hh-GRFd genes were identified on the whole genome level and clustered into five phylogenetic groups with conserved subfamily characteristics. Abundant MeJA-responsive cis-elements were presented in the promoter regions of -. They exhibited a tissue-specific expression variation, and was dominantly expressed in leaves of . Notably, exhibited MeJA-induced expression that correlated temporally with flavonoid accumulation, suggesting that might play a potential role in promoting flavonoid biosynthesis, and overexpression and knockout assay substantiated this conclusion. The finding provides the first transcriptome-wide resource for flavonoid biosynthesis in and identifies the candidate GRF-mediated regulator for flavonoid accumulation.
黄酮类化合物是植物中的关键生物活性化合物，在抵御非生物胁迫中发挥重要防御作用，并参与植物的生长发育。茉莉酸甲酯（MeJA）是一种重要的生长调节剂，可促进多种植物中黄酮类化合物的积累，但MeJA在[具体植物或物种未明确]中的作用仍知之甚少。在本研究中，MeJA处理后黄酮类化合物含量显著增加，并在处理后6小时达到峰值。通过转录组共鉴定出31931个基因，处理后6小时鉴定出6484个差异表达基因（DEGs）。通过基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，DEGs主要富集在苯丙烷生物合成途径中。基于从DEGs推导的假定转录因子，进一步鉴定了生长调节因子（GRF），这是一种潜在地将MeJA信号与黄酮类化合物积累联系起来并参与植物生长和胁迫反应的转录因子。在全基因组水平上共鉴定出20个Hh-GRFd基因，并聚类为五个具有保守亚家族特征的系统发育组。在[基因名称未明确]的启动子区域存在丰富的MeJA响应顺式元件。它们表现出组织特异性表达变化，并且在[植物名称未明确]的叶片中优势表达。值得注意的是，[基因名称未明确]表现出MeJA诱导的表达，其在时间上与黄酮类化合物积累相关，表明[基因名称未明确]可能在促进黄酮类化合物生物合成中发挥潜在作用，过表达和敲除试验证实了这一结论。该发现为[植物名称未明确]中黄酮类化合物生物合成提供了首个全转录组范围的资源，并鉴定了黄酮类化合物积累的候选GRF介导的调节因子。
