Soybean Confers Salt-Alkali Stress Tolerance in Transgenic Plants.

In order to mitigate the reduction in soybean yield caused by soil salinization, a soybean gene, , which promotes plant tolerance to salt-alkali stress, was identified in this study. The STRING database was used to predict the interaction between GmSNF4 and GmPKS4. The gene was experimentally shown to be involved in salt-alkali stress tolerance. Firstly, the yeast two-hybrid technique and bimolecular fluorescence complementation (BiFC) technique were used to confirm the interaction between GmSNF4 and GmPKS4: the AMPK-CBM-CBS1 conserved domain was thereby determined to be the region of the GmSNF4 protein involved in the interaction. Secondly, the gene was induced by salt-alkali stress according to qRT-PCR analysis, and the GmSNF4 protein was localized in the nucleus and cytoplasm. Finally, analysis of 's role in resistance to salt-alkali stress in transgenic soybean plants showed that transgenic lines had better phenotypic, physiological, and stress-related gene expression than non-transgenic soybeans. Thus, may play a significant role in plant salt-alkali stress tolerance.