López-Sierra Carmen, Cos-Terrer José E, Romero-Muñoz Miriam, Pérez-Jiménez Margarita
Department of Plant Biotechnology, Genomics and Breeding, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), 30150 Murcia, Spain.
Plants (Basel). 2025 Jul 18;14(14):2230. doi: 10.3390/plants14142230.
Light influence on shoot regeneration in is a complex interaction that has been studied for the first time. Japanese plum plants were regenerated from calli and seeds of the scion cultivar 'Victoria'. The effect of four different light spectra (white, blue, red, and mixed), along with three 6-benzyladenine (BA) concentrations (1, 1.5, and 2 mg L), was studied in these two sources of explants. Organogenic calli were derived from the base of stem explants of the scion cultivar 'Victoria', whereas cotyledons and embryogenic axis slices were used as seed explants. Calli cultured with 2 mg L of BA and mixed light or 2.5 mg L of BA and control light showed the highest regeneration rates, with no significant differences compared to other treatments. Seed explants exposed to 2.5 mg L of BA and red light exhibited significantly higher organogenesis. In comparison, those in 1.5 mg L of BA with blue light or 2.5 mg L of BA with mixed/control light showed no regeneration. BA concentration did not have a significant effect in the induction of somatic shoots from any explant source. In contrast, a strong interaction between light and BA was noticed. This work presents a protocol that can be applied in transformation and editing research as light spectrum studies continue to advance.
光对李子芽再生的影响是一种复杂的相互作用，这是首次被研究。日本李植株从接穗品种‘维多利亚’的愈伤组织和种子再生而来。在这两种外植体来源中，研究了四种不同光质（白色、蓝色、红色和混合光）以及三种6-苄基腺嘌呤（BA）浓度（1、1.5和2 mg/L）的影响。器官发生愈伤组织源自接穗品种‘维多利亚’茎外植体的基部，而子叶和胚轴切片用作种子外植体。用2 mg/L BA和混合光培养的愈伤组织或2.5 mg/L BA和对照光培养的愈伤组织显示出最高的再生率，与其他处理相比无显著差异。暴露于2.5 mg/L BA和红光下的种子外植体表现出显著更高的器官发生。相比之下，1.5 mg/L BA与蓝光或2.5 mg/L BA与混合/对照光处理下的外植体没有再生。BA浓度对任何外植体来源的体细胞芽诱导均无显著影响。相反，注意到光和BA之间存在强烈的相互作用。随着光谱研究的不断推进，这项工作提出了一种可应用于转化和编辑研究的方案。
