Ricci Angela, Sabbadini Silvia, Prieto Humberto, Padilla Isabel Mg, Dardick Chris, Li Zhijian, Scorza Ralph, Limera Cecilia, Mezzetti Bruno, Perez-Jimenez Margarita, Burgos Lorenzo, Petri Cesar
Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, 60131 Ancona, Italy.
Laboratorio de Biotecnología, La Platina Research Station, Instituto de Investigaciones Agropecuarias, Santa Rosa, La Pintana, Santiago 11610, Chile.
Plants (Basel). 2020 Jul 31;9(8):971. doi: 10.3390/plants9080971.
Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established. Many strategies have been tested in order to obtain an efficient peach transformation system. Despite the amount of time and the efforts invested, the lack of success has significantly limited the utility of peach as a model genetic system for trees, despite its relatively short generation time; small, high-quality genome; and well-studied genetic resources. Additionally, the absence of efficient genetic transformation protocols precludes the application of many biotechnological tools in peach breeding programs. In this review, we provide an overview of research on regeneration and genetic transformation in this species and summarize novel strategies and procedures aimed at producing transgenic peaches. Promising future approaches to develop a robust peach transformation system are discussed, focusing on the main bottlenecks to success including the low efficiency of -mediated transformation, the low level of correspondence between cells competent for transformation and those that have regenerative competence, and the high rate of chimerism in the few shoots that are produced following transformation.
自首次发表关于转化桃植株再生的报道以来，已过去近30年。然而，该物种遗传转化的普遍适用性尚未确立。为了获得高效的桃转化系统，人们已经测试了许多策略。尽管投入了大量时间和精力，但缺乏成功显著限制了桃作为树木模型遗传系统的效用，尽管其世代时间相对较短；基因组小且质量高；遗传资源也得到了充分研究。此外，缺乏有效的遗传转化方案阻碍了许多生物技术工具在桃育种计划中的应用。在这篇综述中，我们概述了该物种再生和遗传转化的研究，并总结了旨在培育转基因桃的新策略和程序。讨论了未来开发强大桃转化系统的有前景的方法，重点关注成功的主要瓶颈，包括介导转化效率低、有转化能力的细胞与具有再生能力的细胞之间的对应水平低，以及转化后产生的少数芽中嵌合体发生率高。
