Stott D I, McLearie J, Marsden H S
Anal Biochem. 1985 Sep;149(2):454-60. doi: 10.1016/0003-2697(85)90597-4.
The design of a gel transfer tank for immunoblotting is described. It is simple and cheap to make, provides a uniform field and uniform transfer over the whole area of the gel, and can easily be adapted for use with any size of gel. It has been used for transfer of proteins from both sodium dodecyl sulfate-polyacrylamide and isoelectric focusing gels to nitrocellulose membranes and its application to the analysis of nuclear proteins is described.
本文描述了一种用于免疫印迹的凝胶转移槽的设计。它制作简单且成本低廉，能在凝胶的整个区域提供均匀的电场和均匀的转移效果，并且可以轻松适配任何尺寸的凝胶。它已被用于将蛋白质从十二烷基硫酸钠 - 聚丙烯酰胺凝胶和等电聚焦凝胶转移到硝酸纤维素膜上，并介绍了其在核蛋白分析中的应用。
