Trotter J A, Samora A, Baca J
Anat Rec. 1985 Sep;213(1):16-25. doi: 10.1002/ar.1092130104.
At the muscle-tendon junction of skeletal muscle fibers the structural interface between muscle cell and connective tissue is amplified by tapering, by indentation, and by surface folding. The precise form taken by the surface folds has been unknown due to a lack of studies on the three-dimensional geometry of the muscle-tendon junction. Analysis of this region by scanning electron microscopy, using conventional preparative techniques, is uninformative because the muscle surface is covered by connective tissue. Removal of the connective tissue from individual murine muscle fibers by incubation of fixed fibers in hot HCl, followed in some instances by treatment with collagenase, permits SEM analysis of the uncovered fiber ends. The muscle fiber end is characterized by surface specializations in the form of anastamotic cylindrical folds. Transmission electron micrographs of cross sections and of serial longitudinal sections of muscle fiber ends confirm that the SEM observations are correct.
在骨骼肌纤维的肌腱连接处，肌肉细胞与结缔组织之间的结构界面通过逐渐变细、凹陷和表面折叠得以放大。由于缺乏对肌腱连接处三维几何结构的研究，表面褶皱的确切形态一直不为人知。使用传统制备技术通过扫描电子显微镜对该区域进行分析并无助益，因为肌肉表面被结缔组织覆盖。通过将固定的纤维在热盐酸中孵育，然后在某些情况下用胶原酶处理，从单个小鼠肌肉纤维上去除结缔组织，从而能够对暴露的纤维末端进行扫描电子显微镜分析。肌肉纤维末端的特征是呈吻合状圆柱形褶皱形式的表面特化结构。肌肉纤维末端的横截面和连续纵向切片的透射电子显微照片证实了扫描电子显微镜的观察结果是正确的。
