Mamut Reyim, Yisilam Gulbar, Adil Guldiyar, Anwar Gulmira
College of Life Sciences and Technology, Xinjiang University, Urumchi, China.
Front Microbiol. 2025 Jul 15;16:1599036. doi: 10.3389/fmicb.2025.1599036. eCollection 2025.
The genus includes terricolous and muscicolous foliose macrolichens that are common and widespread across the majority of continents. The genus is well-defined by the absence of a lower cortex and the presence of a dense arachnoid-tomentose pilema that usually bears anastomosing pale or dark veins with numerous solitary or confluent rhizines. However, the high morphological similarity among its species poses significant taxonomic challenges for accurate identification, often necessitating the use of molecular data. This study presents a comprehensive mitogenomic analysis of 11 species, with the aim of elucidating their genetic structure and taxonomic status. We sequenced and performed assembly of the complete mitochondrial genomes, followed by comprehensive genomic analysis. This analysis revealed that the circular genome length ranged in length from 52,107 bp to 76,353 bp with guanine and cytosine (GC) contents between 26.4 and 27.4%. The mitogenomes encoded 15 protein-coding genes (PCGs), 26-27 transfer RNAs (tRNAs), and 2 ribosomal RNAs (rRNAs). Our analysis revealed a variety of scattered repeats, simple repeats, and tandem repeats, predominantly in intergenic and intron regions. Additionally, we examined codon usage and conducted a synteny analysis within the mitogenomes of species. These findings enhance our understanding of the genetic evolution and phylogenetic relationships within , providing a scientific foundation for future research on the diversity and evolution of lichenized fungi.
该属包括土生和生于藓类植物上的叶状大型地衣，在大多数大陆都很常见且分布广泛。该属的特征明确，缺少下皮层，有浓密的蛛丝状绒毛层，通常带有相互吻合的浅色或深色脉纹以及许多单独或汇合的假根。然而，其物种间高度的形态相似性给准确鉴定带来了重大的分类学挑战，通常需要使用分子数据。本研究对11个物种进行了全面的线粒体基因组分析，旨在阐明它们的遗传结构和分类地位。我们对完整的线粒体基因组进行了测序和组装，随后进行了全面的基因组分析。分析表明，环状基因组长度在52,107 bp至76,353 bp之间，鸟嘌呤和胞嘧啶（GC）含量在26.4%至27.4%之间。线粒体基因组编码15个蛋白质编码基因（PCG）、26 - 27个转运RNA（tRNA）和2个核糖体RNA（rRNA）。我们的分析揭示了各种散布重复序列、简单重复序列和串联重复序列，主要存在于基因间区域和内含子区域。此外，我们检查了密码子使用情况，并在该属物种的线粒体基因组内进行了共线性分析。这些发现增进了我们对该属内遗传进化和系统发育关系的理解，为今后地衣化真菌的多样性和进化研究提供了科学依据。
