Hazlett M J, Little P B, Barnum D A, Maxie M G, Leslie K E, Miller R B
Am J Vet Res. 1985 Nov;46(11):2229-34.
Mammary glands of 6 lactating Holstein cows were inoculated with Haemophilus somnus strain 43826. Three cows developed chronic mastitis and shed bacteria for up to 1 year. Three cows developed acute gangrenous mastitis, with evidence of bacteremia and endotoxemia. Cows with gangrenous mastitis had lower somatic cell counts early after inoculation in affected quarter secretions compared with those in cows that developed chronic mastitis. Cows with gangrenous mastitis developed hypocalcemia, hypoalbuminemia, azotemia, hyperbilirubinemia, mildly increased serum aspartate aminotransferase and creatine kinase activity, and a marked neutropenia with a degenerative left shift. Histopathologic examination of gangrenous quarters revealed edema, necrosis, and vascular thrombosis with few inflammatory cells. A limited survey failed to recover H somnus from dairy cows with clinical mastitis or from mammary secretions from 41 beef cattle at an abattoir.
用睡眠嗜血杆菌43826菌株接种6头泌乳期荷斯坦奶牛的乳腺。3头奶牛发生慢性乳腺炎，细菌排出长达1年。3头奶牛发生急性坏疽性乳腺炎，有菌血症和内毒素血症的证据。与发生慢性乳腺炎的奶牛相比，发生坏疽性乳腺炎的奶牛在接种后早期，患侧乳腺分泌物中的体细胞计数较低。发生坏疽性乳腺炎的奶牛出现低钙血症、低白蛋白血症、氮血症、高胆红素血症、血清天冬氨酸氨基转移酶和肌酸激酶活性轻度升高，以及明显的中性粒细胞减少伴退行性左移。对坏疽乳腺进行组织病理学检查，发现有水肿、坏死和血管血栓形成，炎症细胞较少。一项有限的调查未能从患有临床乳腺炎的奶牛或屠宰场的41头肉牛的乳腺分泌物中分离出睡眠嗜血杆菌。
