Yilma T, Owens S, Adams D S
Am J Vet Res. 1985 Nov;46(11):2360-2.
A serum viral inhibitor (SVI) was isolated from goats and partially characterized. The inhibitor prevented the cytopathic effects of vesicular stomatitis virus, encephalomyocarditis virus, and a caprine herpesvirus, indicating broad antiviral activity. The SVI was distinct from interferon because SVI did not induce an antiviral state in cells (ie, lack of protection of SVI-treated cells from virus challenge). The SVI had activity on heterologous cells, including human, bovine, and ovine cells. The lack of antiviral activity in mouse cells indicated that SVI was not an antibody. Like fibroblast interferon, however, SVI was sensitive to trypsin, was acid stable at pH 2 and 4 C for 4 days, was heat stable at 56 C for 1 hour, and could not be sedimented by centrifugation at 100,000 X g for 4 hours.
从山羊体内分离出一种血清病毒抑制剂（SVI），并对其进行了部分特性鉴定。该抑制剂可预防水疱性口炎病毒、脑心肌炎病毒和一种山羊疱疹病毒的细胞病变效应，表明其具有广泛的抗病毒活性。SVI与干扰素不同，因为SVI不会在细胞中诱导抗病毒状态（即，经SVI处理的细胞对病毒攻击缺乏保护作用）。SVI对包括人、牛和羊细胞在内的异源细胞具有活性。小鼠细胞中缺乏抗病毒活性表明SVI不是抗体。然而，与成纤维细胞干扰素一样，SVI对胰蛋白酶敏感，在pH 2和4℃下4天内酸稳定，在56℃下1小时内热稳定，并且在100,000×g离心4小时不能沉淀。
