Suhadolnik R J, Sawada Y, Flick M B, Reichenbach N L, Mosca J D
Cancer Res. 1983 Nov;43(11):5462-6.
Bleomycin, vincristine, or mitomycin C, when added to HeLa cells simultaneously with human fibroblast interferon (IFN-beta), caused a decrease in cell density and inhibited DNA synthesis compared with HeLa cells treated with IFN-beta alone. However, the IFN-beta-induced antiviral processes were unaffected by the presence of these drugs as determined by in vitro enzyme assays and the development of the antiviral state in the intact HeLa cell. HeLa cells treated with IFN-beta alone or with IFN-beta in combination with bleomycin, vincristine, or mitomycin C were able to induce the double-stranded RNA-dependent adenosine triphosphate:2',5'-oligoadenylic acid adenyltransferase (EC 2.2.2.-) and the double-stranded RNA-dependent protein kinase. Furthermore, the antiviral state as measured by the reduction of plaque-forming units after infection of treated cells (with IFN-beta alone or with IFN-beta plus drugs) with vesicular stomatitis virus was not affected. These results indicate that, under these experimental conditions, the double-stranded RNA-dependent adenosine triphosphate:2',5'-oligoadenylic acid adenyltransferase and protein kinase can be induced by IFN-beta in cells treated with bleomycin, vincristine, or mitomycin C. These cells also develop the antiviral state. These experiments could provide a basis for a careful examination of the effects of interferon on the development of the antiviral state when testing potentially active antineoplastic agents. The possibility that IFN-beta potentiates the cytotoxic effects of bleomycin and mitomycin C on HeLa cells is also discussed.
博来霉素、长春新碱或丝裂霉素C与人类成纤维细胞干扰素（IFN-β）同时添加到HeLa细胞中时，与单独用IFN-β处理的HeLa细胞相比，会导致细胞密度降低并抑制DNA合成。然而，通过体外酶分析以及完整HeLa细胞中抗病毒状态的发展情况确定，这些药物的存在并不影响IFN-β诱导的抗病毒过程。单独用IFN-β或用IFN-β与博来霉素、长春新碱或丝裂霉素C联合处理的HeLa细胞能够诱导双链RNA依赖性三磷酸腺苷：2'，5'-寡腺苷酸腺苷转移酶（EC 2.2.2.-）和双链RNA依赖性蛋白激酶。此外，用单独的IFN-β或IFN-β加药物处理的细胞在感染水疱性口炎病毒后，通过噬斑形成单位减少来衡量的抗病毒状态并未受到影响。这些结果表明，在这些实验条件下，双链RNA依赖性三磷酸腺苷：2'，5'-寡腺苷酸腺苷转移酶和蛋白激酶可由IFN-β在经博来霉素、长春新碱或丝裂霉素C处理的细胞中诱导产生。这些细胞也会形成抗病毒状态。这些实验可为在测试潜在活性抗肿瘤药物时仔细研究干扰素对抗病毒状态发展的影响提供依据。还讨论了IFN-β增强博来霉素和丝裂霉素C对HeLa细胞细胞毒性作用的可能性。
