Wallace B M, Hultén M A
Ann Hum Genet. 1985 Jul;49(3):215-26. doi: 10.1111/j.1469-1809.1985.tb01695.x.
The synaptonemal complexes of oocytes from 16-22 week human fetuses were spread using detergent and silver-stained for examination by light microscopy. Zygotene chromosome synapsis generally begins at the telomeres, without obvious prealignment, and proceeds towards the centromeres. Synapsis is not synchronous and longer bivalents may sometimes be completely paired before shorter ones. At pachytene, when pairing is usually complete, some regions presumed to correspond to the heterochromatic blocks of chromosomes 1.9 and 16 may remain unpaired. Residual univalents are uncommon, and little interlocking is evident at this stage. Desynapsis indicating the beginning of diplotene frequently begins at the telomeres, although there is a general relaxation of pairing throughout the bivalents which become increasingly diffuse as diplotene proceeds. The total synaptonemal complex complement length at pachytene in the female is 519 micron, which is about twice that found in the human male. The implications of these results for genetic mapping are discussed.
使用去污剂铺展来自16 - 22周人类胎儿卵母细胞的联会复合体，并用银染法进行染色，以便通过光学显微镜检查。偶线期染色体联会通常从端粒开始，没有明显的预先排列，然后向着丝粒方向进行。联会不同步，较长的二价体有时可能在较短的二价体完全配对之前就已完全配对。在粗线期，配对通常完成时，一些推测对应于1、9和16号染色体异染色质区的区域可能仍未配对。残余单价体不常见，并且在此阶段几乎没有明显的相互缠绕。表明双线期开始的解联会通常从端粒开始，尽管整个二价体的配对普遍松弛，随着双线期的进行，二价体变得越来越弥散。女性粗线期联会复合体的总长度为519微米，约为人类男性的两倍。讨论了这些结果对基因定位的意义。
